Ouyang Suiyan, Zhang Zhaowei, He Ting, Li Peiwu, Zhang Qi, Chen Xiaomei, Wang Du, Li Hui, Tang Xiaoqian, Zhang Wen
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China.
Toxins (Basel). 2017 Apr 13;9(4):137. doi: 10.3390/toxins9040137.
An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAb) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAb. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.
基于量子点纳米珠（QDNBs）和试纸条开发了一种用于测定大米和花生中总黄曲霉毒素（AFTs）的现场超灵敏定量传感方法。抗AFT单克隆抗体（mAb）为自制并用QDNB标记。在检测线（T线）预包被AFT抗原后，AFT与T线上的AFT抗原和QDNBs-mAb之间进行竞争性免疫反应。在最佳条件下，该方法对AFT反应迅速，在大米和花生基质中的灵敏度分别高达1.4 pg/mL和2.9 pg/mL。放入和取出时间在10分钟内。大米和花生样品基质中AFT的回收率为86.25%至118.0%，相对偏差（RSD）低于12%。通过将该QDNB试纸条与使用加标样品的传统HPLC方法进行比较，进一步验证了该检测方法。因此，该设计为AFT的现场超灵敏定量传感提供了一种潜在的替代方法，也可扩展到用于食品安全的其他化学污染物检测。
