Sönmez Mehmet Fatih, Kılıç Eser, Karabulut Derya, Çilenk KübraTugce, Deligönül Erkan, Dündar Munis
a Department of Histology and Embryology , Faculty of Medicine, Erciyes University .
b Department of Medical Biochemistry , Kayseri , Turkey .
Syst Biol Reprod Med. 2016;62(1):22-30. doi: 10.3109/19396368.2015.1085605. Epub 2015 Nov 13.
Diabetes is known to be associated with erectile dysfunction, retrograde ejaculation, level of testicular hormone, and a decrease in semen quality, respectively. In this project, we aimed to investigate at the molecular level, the effects of NOS on testes pathology in diabetes and examine the effects of pentoxifylline on healing. A total of 50 Wistar albino male rats were divided into five groups: Group I control; Group II only diabetes; Group III and IV diabetes + pentoxifylline; Group V only pentoxifylline. Group III rats received 50 mg/kg/day pentoxifylline during two months. In comparison, Group IV rats received saline in the first month followed by 50 mg/kg/day of pentoxifylline for the following month. NOS expression in testicular tissue was assessed using qRT-PCR, western blot, and immunohistochemistry. The mean seminiferous tubule diameter, Johnsen's testicular biopsy score, and serum testosterone levels decreased compared to controls. In contrast, the number of apoptotic cells, the levels of nNOS, iNOS and eNOS mRNA, and protein increased when compared to the control. Upon pentoxifylline therapy NOS decreased suggesting that it contributes to this damage and treatment with pentoxifylline may be effective in reversing this damage.
众所周知，糖尿病分别与勃起功能障碍、逆行射精、睾丸激素水平以及精液质量下降有关。在本项目中，我们旨在从分子水平研究一氧化氮合酶（NOS）对糖尿病睾丸病理的影响，并考察己酮可可碱对愈合的作用。总共50只Wistar白化雄性大鼠被分为五组：第一组为对照组；第二组仅患糖尿病；第三组和第四组为糖尿病 + 己酮可可碱组；第五组仅给予己酮可可碱。第三组大鼠在两个月内每天接受50毫克/千克的己酮可可碱。相比之下，第四组大鼠在第一个月接受生理盐水，在接下来的一个月接受每天50毫克/千克的己酮可可碱。使用定量逆转录聚合酶链反应（qRT-PCR）、蛋白质免疫印迹法和免疫组织化学法评估睾丸组织中NOS的表达。与对照组相比，生精小管平均直径、约翰森睾丸活检评分和血清睾酮水平降低。相反，与对照组相比，凋亡细胞数量、神经元型NOS（nNOS）、诱导型NOS（iNOS）和内皮型NOS（eNOS）的信使核糖核酸（mRNA）及蛋白质水平增加。经己酮可可碱治疗后，NOS降低，表明其与这种损伤有关，用己酮可可碱治疗可能有效逆转这种损伤。
