Orman Dogan, Vardi Nigar, Ates Burhan, Taslidere Elif, Elbe Hulya
Inonu University, Faculty of Medicine, Department of Histology and Embryology, Malatya, Turkey.
Inonu University, Faculty of Science and Art, Department of Chemistry, Malatya, Turkey.
Tissue Cell. 2015 Jun;47(3):284-90. doi: 10.1016/j.tice.2015.03.006. Epub 2015 Apr 2.
This study aimed to investigate the effect of aminoguanidine (AG) against testicular damage streptozotocin (STZ) induced diabetes. Thirty two rats were separated into four groups: control, AG, STZ and STZ+AG. In the STZ group, 12.5±1.3% of tubules were seen as containing sloughed spermatogenic cells into the lumen, 28.7±1.8% of tubules were atrophic, 46.2±2.1% of tubules were degenerative and 8.5±0.9% of tubules contained giant cells. Statistically, the affected tubule number was significantly lower in the STZ+AG group than in the STZ group. Intensely stained caspase-3 cells showed a statistically significant increase in the STZ group, while it decreased in the STZ+AG group. The enzyme activities of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) level decreased and the level of malondialdehyde (MDA) and nitric oxide (NO) increased in the STZ group, while AG treated diabetic rats showed an increase of CAT, SOD activity and GSH level and a decrease in MDA and NO levels. This study shows that the oxidative stress, increased NO level and apoptotic cell death play an important role in diabetic rat testicular damage and that AG treatment of diabetic rats results in protection of spermatogenic cells against oxidative stress and apoptotic cell death.
本研究旨在探讨氨基胍（AG）对链脲佐菌素（STZ）诱导的糖尿病所致睾丸损伤的影响。32只大鼠被分为四组：对照组、AG组、STZ组和STZ + AG组。在STZ组中，可见12.5±1.3%的曲细精管内有生精细胞脱落至管腔，28.7±1.8%的曲细精管萎缩，46.2±2.1%的曲细精管变性，8.5±0.9%的曲细精管含有巨细胞。统计学分析显示，STZ + AG组的受损曲细精管数量显著低于STZ组。在STZ组中，caspase - 3细胞染色强度显著增加，而在STZ + AG组中则降低。STZ组中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）的酶活性及谷胱甘肽（GSH）水平降低，丙二醛（MDA）和一氧化氮（NO）水平升高，而经AG治疗的糖尿病大鼠CAT、SOD活性及GSH水平升高，MDA和NO水平降低。本研究表明，氧化应激、NO水平升高及凋亡性细胞死亡在糖尿病大鼠睾丸损伤中起重要作用，而对糖尿病大鼠进行AG治疗可保护生精细胞免受氧化应激和凋亡性细胞死亡的影响。
