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用于检测致病性小肠结肠炎耶尔森菌和假结核耶尔森菌菌株的酶免疫测定法的评估

Evaluation of enzyme immunoassay for the detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis strains.

作者信息

Kaneko S, Maruyama T

机构信息

Department of Food Hygiene and Nutrition, Tokyo Metropolitan Research Laboratory of Public Health, Japan.

出版信息

J Clin Microbiol. 1989 Apr;27(4):748-51. doi: 10.1128/jcm.27.4.748-751.1989.

DOI:10.1128/jcm.27.4.748-751.1989
PMID:2656747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267410/
Abstract

To determine the virulence plasmid-harboring strains of Yersinia enterocolitica, we prepared antiserum against plasmid-encoded proteins of Y. enterocolitica serotype O3 and carried out an enzyme immunoassay (EIA) against temperature-inducible released proteins. This serum reacted with proteins released from not only a Y. enterocolitica serotype O3 strain but also Y. enterocolitica serotype O5:27, O8, and O9 and Y. pseudotuberculosis serotype 1b, 2a, 2b, 2c, 3, 4a, 4b, 5a, 5b, 6, 7, and 8 strains, which all harbored plasmids. Plasmid-cured Y. enterocolitica and Y. pseudotuberculosis strains did not react in the EIA, nor did nonpathogenic Y. enterocolitica strains or Y. frederiksenii, Y. intermedia, and Y. kristensenii strains. These observations demonstrated that this EIA was useful for determining whether the isolated Yersinia strains were pathogenic or not.

摘要

为了确定携带毒力质粒的小肠结肠炎耶尔森菌菌株，我们制备了针对小肠结肠炎耶尔森菌O3血清型质粒编码蛋白的抗血清，并对温度诱导释放的蛋白进行了酶免疫测定（EIA）。该血清不仅与小肠结肠炎耶尔森菌O3血清型菌株释放的蛋白发生反应，还与小肠结肠炎耶尔森菌O5:27、O8和O9血清型菌株以及假结核耶尔森菌1b、2a、2b、2c、3、4a、4b、5a、5b、6、7和8血清型菌株释放的蛋白发生反应，这些菌株均携带质粒。质粒消除的小肠结肠炎耶尔森菌和假结核耶尔森菌菌株在EIA中不发生反应，非致病性小肠结肠炎耶尔森菌菌株或费氏耶尔森菌、中间耶尔森菌和克氏耶尔森菌菌株也不发生反应。这些观察结果表明，该EIA可用于确定分离出的耶尔森菌菌株是否具有致病性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50d/267410/38243c8c476d/jcm00064-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50d/267410/25f508277ece/jcm00064-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50d/267410/38243c8c476d/jcm00064-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50d/267410/25f508277ece/jcm00064-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a50d/267410/38243c8c476d/jcm00064-0168-a.jpg

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本文引用的文献

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Plasmid-mediated tissue invasiveness in Yersinia enterocolitica.小肠结肠炎耶尔森菌中质粒介导的组织侵袭性
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