展示小肠结肠炎耶尔森菌质粒相关毒力克隆差异菌落形态的简易方法。
Simple method for demonstration of differential colony morphology of plasmid-associated virulent clones of Yersinia enterocolitica.
作者信息
Mazigh D, Alonso J M, Mollaret H H
出版信息
J Clin Microbiol. 1983 Mar;17(3):555-7. doi: 10.1128/jcm.17.3.555-557.1983.
Abstract
Virulent (plasmid-associated) strains of Yersinia enterocolitica grown on RPMI 1640 agar (RPMI-1640 medium [Flow Laboratories] with 40 mM HEPES and 1.5% [wt/vol] purified agar [Difco]) dissociated into small and large colonies. The autoagglutination test (a marker of virulence) is regularly positive with small colonies and negative with large colonies. Avirulent Y. enterocolitica strains gave only large colonies on RPMI 1640 agar.
摘要
在RPMI 1640琼脂（含40 mM HEPES和1.5% [重量/体积] 纯化琼脂 [Difco] 的RPMI-1640培养基 [Flow Laboratories]）上生长的肠炎耶尔森菌的强毒株（质粒相关）会解离成小菌落和大菌落。自凝试验（一种毒力标志物）在小菌落中通常呈阳性，在大菌落中呈阴性。无毒力的肠炎耶尔森菌菌株在RPMI 1640琼脂上只产生大菌落。
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