用于增强单细胞蛋白质免疫印迹的水凝胶孔径调节
Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.
作者信息
Duncombe Todd A, Kang Chi-Chih, Maity Santanu, Ward Toby M, Pegram Mark D, Murthy Niren, Herr Amy E
机构信息
Department of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.
Division of Oncology, Department of Medicine, Stanford University, Stanford, California, CA 94305, USA.
出版信息
Adv Mater. 2016 Jan 13;28(2):327-334. doi: 10.1002/adma.201503939. Epub 2015 Nov 16.
Abstract
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.
摘要
孔梯度微凝胶阵列能够在1毫米的分离距离内，对分子量范围广泛（25 - 289 kDa）的靶标进行数千次并行的高分辨率单细胞蛋白质电泳分离。双交联水凝胶有助于电泳后凝胶孔的扩张，以实现高效且均匀的免疫探测。这种光图案化、光激活且酸可膨胀的水凝胶是单细胞蛋白质分析的基础，在此用于人类乳腺活检中与癌蛋白相关的信号传导分析。
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