Wu Xiangbing, Yu Jun, Gao Guilin, Wang Xin, Liu Yang, Zhu Shengrong, Gong Zhongjian
Department of Stomatology, Wuxi People's Hospital, Nanjing Medical University, Wuxi, China.
Center of Stomatology, Central Hospital of Enshi Autonomous Prefecture, Enshi, China.
PLoS One. 2015 Nov 16;10(11):e0143009. doi: 10.1371/journal.pone.0143009. eCollection 2015.
Upregulation of heparanase has been reported in an increasing number of human cancer tissues. However, the level of salivary heparanase and its clinical significance in patients with salivary gland tumors remain unclear.
Salivary heparanase levels in patients with salivary gland tumors were detected using enzyme-linked immunosorbent assays (ELISAs) and the clinical significance was evaluated by analyzing the correlations among salivary heparanase levels, clinicopathological parameters, and clinical outcomes.
The levels of salivary heparanase were significantly higher in patients with malignant salivary gland tumors than in benign tumors and normal controls (P<0.0001). High salivary heparanase levels were positively correlated with increased lymph node metastasis (P = 0.0235) and poorer tumor node metastasis stage (TNM) (P = 0.0183). Survival analyses revealed that high salivary heparanase levels were associated with worse overall survival (P = 0.0023) and disease-free survival (DFS) (P = 0.0025).
The study shows that salivary heparanase levels, as detected by the ELISAs, can be used to diagnose and provide an accurate prognosis for malignant salivary gland tumors. Salivary heparanase level was an independent predictor in patients with malignant salivary gland tumors.
越来越多的人类癌症组织中已报道硫酸乙酰肝素酶上调。然而，唾液中硫酸乙酰肝素酶水平及其在涎腺肿瘤患者中的临床意义仍不清楚。
采用酶联免疫吸附测定（ELISA）检测涎腺肿瘤患者唾液中硫酸乙酰肝素酶水平，并通过分析唾液中硫酸乙酰肝素酶水平、临床病理参数和临床结局之间的相关性来评估其临床意义。
恶性涎腺肿瘤患者唾液中硫酸乙酰肝素酶水平显著高于良性肿瘤患者和正常对照（P<0.0001）。唾液中硫酸乙酰肝素酶水平高与淋巴结转移增加（P = 0.0235）和肿瘤淋巴结转移分期（TNM）较差（P = 0.0183）呈正相关。生存分析显示，唾液中硫酸乙酰肝素酶水平高与总生存期较差（P = 0.0023）和无病生存期（DFS）较差（P = 0.0025）相关。
该研究表明，ELISA检测的唾液中硫酸乙酰肝素酶水平可用于诊断恶性涎腺肿瘤并提供准确的预后。唾液中硫酸乙酰肝素酶水平是恶性涎腺肿瘤患者的独立预测指标。
