The School of Chemical Engineering, The University of Queensland, Brisbane, Australia.
J Immunol Methods. 2011 Oct 28;373(1-2):19-25. doi: 10.1016/j.jim.2011.07.013. Epub 2011 Jul 27.
Cardiovascular disease is the leading cause of death in the world. Human C-reactive protein (CRP) has been used in the risk assessment of coronary events. Human saliva mirrors the body's health and well-being and is non-invasive, easy to collect and ideal for third world countries as well as for large patient screening. The aim was to establish a saliva CRP reference range and to demonstrate the clinical utility of salivary CRP levels in assessing the coronary events in a primary health care setting.
We have used a homogeneous bead based assay to detect CRP levels in human saliva. We have developed a rapid 15 min (vs 90 min), sequential, one-step assay to detect CRP in saliva. Saliva was collected from healthy volunteers (n=55, ages 20-70 years) as well as from cardiac patients (n=28, ages 43-86 years).
The assay incubation time was optimised from 90 min to 15 min and generated a positive correlation (n=29, range 10-2189 pg/mL, r2=0.94; Passing Bablok slope 0.885, Intercept 0, p>0.10), meaning we could decrease the incubation time and produce equivalent results with confidence. The mean CRP level in the saliva of healthy human volunteers was 285 pg/mL and in cardiac patients was 1680 pg/mL (p<0.01). Analysis of CRP concentrations in paired serum and saliva samples from cardiac patients gave a positive correlation (r2=0.84, p<0.001) and the salivary CRP concentration capable of distinguishing healthy from diseased patients.
The results suggest that this minimally invasive, rapid and sensitive assay will be useful in large patient screening studies for risk assessment of coronary events.
心血管疾病是世界上的主要死因。人类 C 反应蛋白（CRP）已被用于评估冠心病事件的风险。人类唾液反映了身体的健康和整体状况，并且具有非侵入性、易于采集等特点，因此不仅适用于第三世界国家，也适用于大规模患者筛查。本研究旨在建立唾液 CRP 的参考范围，并证明唾液 CRP 水平在基层医疗保健环境中评估冠心病事件的临床应用价值。
我们使用均相 bead 检测法检测人类唾液中的 CRP 水平。我们开发了一种快速的 15 分钟（而不是 90 分钟）、连续的一步法检测唾液中的 CRP。采集了健康志愿者（n=55，年龄 20-70 岁）和心脏病患者（n=28，年龄 43-86 岁）的唾液样本。
优化了检测的孵育时间，从 90 分钟缩短至 15 分钟，并生成了正相关（n=29，范围 10-2189 pg/mL，r2=0.94；Passing Bablok 斜率 0.885，截距 0，p>0.10），这意味着我们可以缩短孵育时间并充满信心地获得等效结果。健康志愿者的唾液平均 CRP 水平为 285 pg/mL，心脏病患者为 1680 pg/mL（p<0.01）。对心脏病患者配对的血清和唾液样本中的 CRP 浓度进行分析，结果显示呈正相关（r2=0.84，p<0.001），并且唾液 CRP 浓度能够区分健康和患病患者。
这些结果表明，这种微创、快速和敏感的检测方法将有助于进行大规模患者筛查研究，以评估冠心病事件的风险。
