人免疫缺陷病毒1型gag多蛋白前体在酿酒酵母中的N-肉豆蔻酰化作用
N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae.
作者信息
Bathurst I C, Chester N, Gibson H L, Dennis A F, Steimer K S, Barr P J
机构信息
Chiron Corporation, Emeryville, California 94608-2916.
出版信息
J Virol. 1989 Jul;63(7):3176-9. doi: 10.1128/JVI.63.7.3176-3179.1989.
A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.
一个精确编码1型人类免疫缺陷病毒gag前体（Pr53gag）502个氨基酸的半合成基因在酿酒酵母中得以表达。对重组Pr53gag的氨基酸序列分析表明其氨基末端完全被封闭。用[3H]肉豆蔻酸对Pr53gag进行标记显示，与从病毒感染细胞中分离得到的Pr53gag一样，酵母来源的蛋白在第二个氨基酸残基甘氨酸上发生了去甲硫氨酸化和N-肉豆蔻酰化。
Zotero 插件