Takashima Satoshi, Shibata Sanae, Yamada Kazuto, Ogawa Mizuho, Nishii Naohito, Kitagawa Hitoshi
Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Vet Anaesth Analg. 2016 Jul;43(4):379-87. doi: 10.1111/vaa.12319. Epub 2015 Nov 17.
To evaluate the effect of intravenous infusion of amino acids on the prevention of hypothermia during anaesthesia in dogs.
Randomized experimental trial.
Seven healthy Beagle dogs.
Four concentrations of amino acids were prepared with a 10% amino acid solution and an acetated Ringer's solution, and dogs were infused with each of the solutions at 1 week intervals. Dogs were infused with amino acid solution at 12 mL kg(-1) hour(-1) for 60 minutes before and for 60 minutes after induction of anaesthesia. Acetated Ringer's solution was infused at the same rate for the remaining 60 minutes of anaesthesia. The infusion treatments were: 1) A0, nutrient-free acetated Ringer's solution; 2) A6, 0.6 g kg(-1) hour(-1) ; 3) A9, 0.9 g kg(-1) hour(-1) ; and 4) A12, 1.2 g kg(-1) hour(-1) . Rectal temperature (RT), heart rate (HR), mean arterial pressure (MAP), blood insulin, glucose, urea nitrogen (BUN) and creatinine concentrations, and time to extubation were measured.
Before anaesthesia, RT was not affected by amino acid infusion. RT decreased progressively during anaesthesia and the absolute values of RT from 30 to 120 minutes were significantly higher in A12 than in A0 (p < 0.05). Reductions in HR and MAP during anaesthesia were attenuated by amino acid infusion in a dose-dependent manner. Plasma insulin concentration was significantly higher in A12 than in A0 during amino acid infusion and the increase in insulin concentration was greater during than before anaesthesia. BUN increased during amino acid infusion in a dose- and time-dependent fashion. Time until extubation was shorter in A12 than in A0.
Amino acids infused at 1.2 g kg(-1) hour(-1) in dogs attenuated the decrease in RT, HR, and MAP during anaesthesia, and induced a significant increase in plasma insulin concentration.
评估静脉输注氨基酸对预防犬麻醉期间体温过低的效果。
随机实验性试验。
7只健康的比格犬。
用10%氨基酸溶液和醋酸林格氏液配制4种浓度的氨基酸溶液，每隔1周给犬输注每种溶液。在麻醉诱导前60分钟和诱导后60分钟，以12 mL·kg⁻¹·小时⁻¹的速度给犬输注氨基酸溶液。在麻醉的其余60分钟内，以相同速度输注醋酸林格氏液。输注治疗方案为：1）A0，无营养的醋酸林格氏液；2）A6，0.6 g·kg⁻¹·小时⁻¹；3）A9，0.9 g·kg⁻¹·小时⁻¹；4）A12，1.2 g·kg⁻¹·小时⁻¹。测量直肠温度（RT）、心率（HR）、平均动脉压（MAP）、血液胰岛素、葡萄糖、尿素氮（BUN）和肌酐浓度，以及拔管时间。
麻醉前，RT不受氨基酸输注的影响。麻醉期间RT逐渐下降，A12组在30至120分钟时的RT绝对值显著高于A0组（p < 0.05）。麻醉期间HR和MAP的降低通过氨基酸输注以剂量依赖性方式得到缓解。在输注氨基酸期间，A12组的血浆胰岛素浓度显著高于A0组，且胰岛素浓度在麻醉期间的升高大于麻醉前。在输注氨基酸期间，BUN以剂量和时间依赖性方式增加。A12组的拔管时间比A0组短。
以1.2 g·kg⁻¹·小时⁻¹的速度给犬输注氨基酸可减轻麻醉期间RT、HR和MAP的下降，并使血浆胰岛素浓度显著升高。
