Long Ping, Cui Zhanhu, Wang Yingli, Zhang Chunhong, Zhang Na, Li Minhui, Xiao Peigen
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China ; Baotou Medical College, Baotou 014060, China.
Baotou Medical College, Baotou 014060, China.
Acta Pharm Sin B. 2014 Jun;4(3):227-37. doi: 10.1016/j.apsb.2014.02.006. Epub 2014 Mar 24.
Non-Camellia tea is a part of the colorful Chinese tea culture, and is also widely used as beverage and medicine in folk for disease prevention and treatment. In this study, 37 samples were collected, including 33 kinds of non-Camellia teas and 4 kinds of teas (Camellia). Traditional functions of non-Camellia teas were investigated. Furthermore, non-Camellia teas of original plants were characterized and identified by molecular methods. Four candidate regions (rbcL, matK, ITS2, psbA-trnH) were amplified by polymerase chain reaction. In addition, DNA barcodes were used for the first time to discriminate the commercial non-Camellia tea and their adulterants, and to evaluate their safety. This study showed that BLASTN and the relevant phylogenetic tree are efficient tools for identification of the commercial non-Camellia tea and their adulterants. However, some sequences from original plants have not been found and there is a limitation of sequence number of original plants in GenBank. Submitting more original plant sequences to the GenBank will be helpful for evaluating the safety of non-Camellia teas.
非山茶属茶叶是丰富多彩的中国茶文化的一部分，在民间也被广泛用作预防和治疗疾病的饮品和药物。在本研究中，收集了37个样本，包括33种非山茶属茶叶和4种山茶属茶叶。对非山茶属茶叶的传统功能进行了研究。此外，通过分子方法对非山茶属茶叶的原植物进行了特征鉴定。通过聚合酶链反应扩增了四个候选区域（rbcL、matK、ITS2、psbA-trnH）。此外，首次使用DNA条形码鉴别市售非山茶属茶叶及其掺假品，并评估其安全性。本研究表明，BLASTN和相关系统发育树是鉴定市售非山茶属茶叶及其掺假品的有效工具。然而，尚未找到一些原植物的序列，并且GenBank中原植物的序列数量有限。向GenBank提交更多原植物序列将有助于评估非山茶属茶叶的安全性。
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