Li Chong, Tan Fang, Yang Jianjun, Yang Yue, Gou Yuting, Li Shuting, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Antioxidants (Basel). 2019 Sep 8;8(9):381. doi: 10.3390/antiox8090381.
As a traditional Chinese medicinal drink, , a local tea from Xinjiang, China, is favored for its rich flavor and biological functionality. This study looked at aging mice induced by d-galactose to determine the in vivo anti-aging effect of tea extracts (AVTEs) and its bioactive components. We evaluated the weight of major organs (via organ index) and pathological changes in the liver. We also detailed the effects of AVTE (250 mg/kg in the low dose group, 500 mg/kg in the high dose group) on biochemical parameters (malondialdehyde, superoxide dismutase, glutathione, glutathione peroxidase, catalase, total antioxidant capacity, and nitric oxide) and cytokines (IL-6, IL-12, TNF-α and IL-1β) in the serum of aging mice. We investigated the anti-aging effects of AVTE in d-galactose-induced aging mice via quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) assay. In addition, we analyzed the biological components of AVTEs by high performance liquid chromatography (HPLC). The results were remarkable, suggesting that AVTE significantly improved d-galactose-induced aging mice, with the high dose group showing the best results among other groups. ATVE can effectively alleviate hepatocyte edema, as well as inflammatory cell infiltration and injury in mice, induce a protective effect via up-regulation of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) antioxidant related factors, and play an important role in the up-regulation of anti-inflammatory factors (IL-10) and the down-regulation of pro-inflammatory factors (IL-6, TNF-α and IL-1β). At the same time, HPLC analysis showed that AVTEs contain neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, astragalin, isochlorogenic acid C, rosmarinic acid, and trans-cinnamic acid. Thus, AVTE appears to be an effectively functional drink due to its rich functional components and anti-aging activities.
作为一种中国传统药茶， 是一种源自中国新疆的地方茶，因其浓郁的风味和生物功能而备受青睐。本研究观察了由D-半乳糖诱导衰老的小鼠，以确定 茶提取物（AVTEs）及其生物活性成分的体内抗衰老作用。我们评估了主要器官的重量（通过器官指数）以及肝脏的病理变化。我们还详细研究了AVTE（低剂量组为250mg/kg，高剂量组为500mg/kg）对衰老小鼠血清中生化参数（丙二醛、超氧化物歧化酶、谷胱甘肽、谷胱甘肽过氧化物酶、过氧化氢酶、总抗氧化能力和一氧化氮）和细胞因子（IL-6、IL-12、TNF-α和IL-1β）的影响。我们通过定量实时逆转录聚合酶链反应（RT-qPCR）分析研究了AVTE对D-半乳糖诱导的衰老小鼠的抗衰老作用。此外，我们通过高效液相色谱（HPLC）分析了AVTEs的生物成分。结果显著，表明AVTE显著改善了D-半乳糖诱导的衰老小鼠，高剂量组在其他组中表现最佳。ATVE可以有效减轻小鼠肝细胞水肿以及炎性细胞浸润和损伤，通过上调谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等抗氧化相关因子发挥保护作用，并在抗炎因子（IL-10）上调和促炎因子（IL-6、TNF-α和IL-1β）下调中发挥重要作用。同时，HPLC分析表明，AVTEs含有新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A、黄芪苷、异绿原酸C、迷迭香酸和反式肉桂酸。因此，由于其丰富的功能成分和抗衰老活性，AVTE似乎是一种有效的功能性饮品。 （注：原文中茶名处有缺失内容）
