a Swine Science and Technology Center , Gyeongnam National University of Science and Technology , Jinju , South Korea.
b Dasan Pig Breeding Co. , Namwon-si , South Korea.
Anim Biotechnol. 2018;29(4):301-308. doi: 10.1080/10495398.2017.1395345. Epub 2017 Dec 4.
Litter size is among the most important traits in swine breeding. However, information on the genetics of litter size in pigs is lacking. In this study, we identified single nucleotide polymorphisms (SNPs) in the insulin-like growth factor binding protein 2 and 3 (IGFBP2 and IGFBP3) genes in Berkshire pigs and analyzed their association with litter size traits. The IGFBP2 SNP was located on chromosome 15 intron 2 (455, A > T) and the IGFBP3 SNP was on chromosome 18 intron 2 (53, A > G). The AT type of IGFBP2 and the GG type of IGFBP3 had the highest values for all litter size traits including total number born (TNB), number of pigs born alive, and breeding value according to TNB. Homozygous GG pigs expressed higher levels of IGFBP3 mRNA in the endometrium than pigs of other genotypes, and a positive correlation was observed between litter size traits and IGFBP3 but not IGFBP2 expression level. These results suggest that SNPs in the IGFBP2 and the IGFBP3 gene are useful biomarkers for increasing the reproductive productivity of Berkshire pigs.
产仔数是猪种选育中最重要的性状之一。然而，关于猪产仔数的遗传学信息还很缺乏。本研究在伯克夏猪中鉴定了胰岛素样生长因子结合蛋白 2 和 3（IGFBP2 和 IGFBP3）基因中的单核苷酸多态性（SNP），并分析了它们与产仔数性状的关系。IGFBP2 SNP 位于第 15 号染色体内含子 2（455，A>G），IGFBP3 SNP 位于第 18 号染色体内含子 2（53，A>G）。IGFBP2 的 AT 型和 IGFBP3 的 GG 型在所有产仔数性状中具有最高值，包括总产仔数（TNB）、活产仔数和 TNB 的繁殖价值。与其他基因型的猪相比，IGFBP3 基因纯合 GG 型猪的子宫内膜中 IGFBP3 mRNA 表达水平更高，并且产仔数性状与 IGFBP3 表达水平呈正相关，而与 IGFBP2 表达水平无关。这些结果表明，IGFBP2 和 IGFBP3 基因中的 SNP 是提高伯克夏猪繁殖生产力的有用生物标志物。
