Ye Jian-Hong, Liu Meng-Hua, Zhang Xu-Lin, He Jing-Yu
Department of Endocrinology, Foshan Hospital of Traditional Chinese Medicine, Foshan 528000, China.
Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Int J Mol Sci. 2015 Nov 13;16(11):27252-69. doi: 10.3390/ijms161126021.
Protective effect of Hedyotis diffusa (H. diffusa) Willd against lipopolysaccharide (LPS)-induced renal inflammation was evaluated by the productions of cytokines and chemokine, and the bioactive constituents of H. diffusa were detected by the ultra-fast liquid chromatography-diode array detector-quadrupole-time of flight mass spectrometry (UFLC-DAD-Q-TOF-MS/MS) method. As the results showed, water extract of H. diffusa (equal to 5.0 g/kg body weight) obviously protected renal tissues, significantly suppressed the productions of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein (MCP)-1, as well as significantly promoted the production of IL-10 in serum and renal tissues. According the chemical profiles of H. diffusa, flavonoids, iridoid glycosides and anthraquinones were greatly detected in serum from H. diffusa extract treatment mice. Two main chemotypes, including eight flavonoids and four iridoid glycosides were found in renal tissues from H. diffusa extract treatment mice. The results demonstrated that water extract of H. diffusa had protective effect on renal inflammation, which possibly resulted from the bioactive constituents consisting of flavonoids, iridoids and anthraquinones.
通过细胞因子和趋化因子的产生来评估白花蛇舌草对脂多糖（LPS）诱导的肾脏炎症的保护作用，并采用超快速液相色谱-二极管阵列检测器-四极杆-飞行时间质谱（UFLC-DAD-Q-TOF-MS/MS）法检测白花蛇舌草的生物活性成分。结果显示，白花蛇舌草水提取物（相当于5.0 g/kg体重）对肾组织有明显的保护作用，显著抑制肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6和单核细胞趋化蛋白（MCP）-1的产生，并显著促进血清和肾组织中IL-10的产生。根据白花蛇舌草的化学图谱，在白花蛇舌草提取物处理小鼠的血清中大量检测到黄酮类、环烯醚萜苷和蒽醌类化合物。在白花蛇舌草提取物处理小鼠的肾组织中发现了两种主要化学类型，包括八种黄酮类化合物和四种环烯醚萜苷。结果表明，白花蛇舌草水提取物对肾脏炎症具有保护作用，这可能是由黄酮类、环烯醚萜类和蒽醌类生物活性成分所致。
