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麻风分枝杆菌18千道尔顿抗原的T细胞识别

T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae.

作者信息

Dockrell H M, Stoker N G, Lee S P, Jackson M, Grant K A, Jouy N F, Lucas S B, Hasan R, Hussain R, McAdam K P

机构信息

Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, United Kingdom.

出版信息

Infect Immun. 1989 Jul;57(7):1979-83. doi: 10.1128/iai.57.7.1979-1983.1989.

DOI:10.1128/iai.57.7.1979-1983.1989
PMID:2659530
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313830/
Abstract

The 18-kilodalton (kDa) antigen of Mycobacterium leprae was expressed as a fusion protein with a 2-kDa leader peptide and used in proliferation assays with peripheral blood cells. Fifty percent of untreated tuberculoid leprosy patients and 93% of long-term leprosy contacts responded to the recombinant protein in lymphocyte transformation tests. Comparison of the stimulation indices in the two groups showed that the contacts responded more strongly than the tuberculoid leprosy patients. Seventy percent of Mycobacterium bovis BCG-vaccinated European donors responded, although with low stimulation indices. The isolation of 18-kDa antigen-responsive T-cell lines from a BCG-vaccinated British donor confirmed that the 18-kDa antigen contains at least one cross-reactive epitope. These results indicate that the 18-kDa protein is an important antigen in the immune response to leprosy.

摘要

麻风分枝杆菌的18千道尔顿（kDa）抗原与一个2 kDa的前导肽融合表达为融合蛋白，并用于外周血细胞的增殖试验。50%未经治疗的结核样型麻风患者和93%的长期麻风接触者在淋巴细胞转化试验中对重组蛋白有反应。两组刺激指数的比较表明，接触者的反应比结核样型麻风患者更强。70%接种牛分枝杆菌卡介苗的欧洲供者有反应，尽管刺激指数较低。从一名接种卡介苗的英国供者中分离出对18 kDa抗原反应的T细胞系，证实18 kDa抗原至少含有一个交叉反应表位。这些结果表明，18 kDa蛋白是麻风免疫反应中的一种重要抗原。

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本文引用的文献

1
Selective primary health care: strategies for control of disease in the developing world. V. Leprosy.
Rev Infect Dis. 1983 Jul-Aug;5(4):765-80. doi: 10.1093/clinids/5.4.765.
2
Easy identification of cDNA clones.
EMBO J. 1983;2(10):1791-4. doi: 10.1002/j.1460-2075.1983.tb01659.x.
3
Fluorescent leprosy antibody absorption (FLA-ABS) test for detecting subclinical infection with Mycobacterium leprae.
Int J Lepr Other Mycobact Dis. 1980 Jun;48(2):109-19.
4
Growing points in leprosy research. 3. Immunological detection of sub-clinical infection in leprosy.
Lepr Rev. 1974 Mar;45(1):22-30. doi: 10.5935/0305-7518.19740006.
5
Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family.
Mol Cell Biol. 1985 Dec;5(12):3417-28. doi: 10.1128/mcb.5.12.3417-3428.1985.
6
Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae.
Nature. 1985;316(6027):450-2. doi: 10.1038/316450a0.
7
Characterization of the 36 K antigen of Mycobacterium leprae.
Lepr Rev. 1986 Dec;57 Suppl 2:77-81. doi: 10.5935/0305-7518.19860057.
8
The characterization and immunoreactivity of a 70 kD protein common to Mycobacterium leprae and Mycobacterium bovis (BCG).
Lepr Rev. 1986 Dec;57 Suppl 2:67-75.
9
Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli.
Nature. 1986;319(6048):63-6. doi: 10.1038/319063a0.
10
Stress proteins are immune targets in leprosy and tuberculosis.
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267-70. doi: 10.1073/pnas.85.12.4267.

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