Tim Carla Roberta, Bossini Paulo Sérgio, Kido Hueliton Wilian, Malavazi Iran, von Zeska Kress Marcia Regina, Carazzolle Marcelo Falsarella, Parizotto Nivaldo Antonio, Rennó Ana Cláudia
Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil.
Federal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil.
J Photochem Photobiol B. 2016 Jan;154:8-15. doi: 10.1016/j.jphotobiol.2015.10.028. Epub 2015 Oct 31.
The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830nm, 30mW, 2,8J, 94seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, IL1, 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36h and 3days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing.
在骨缺损实验模型中，研究了低强度激光疗法（LLLT）后的骨愈合过程以及炎症和血管生成基因的表达。将60只Wistar大鼠分为对照组和激光组（830nm，30mW，2.8J，94秒）。组织病理学分析表明，LLLT能够调节骨缺损区域的炎症过程，并能促使肉芽组织和新形成的骨组织更早沉积。微阵列分析显示，LLLT在36小时和3天时使与炎症过程相关的基因（MMD、PTGIR、PTGS2、Ptger2、IL1、IL6、IL8、IL18）和血管生成基因（FGF14、FGF2、ANGPT2、ANGPT4和PDGFD）上调，随后在第7天基因表达下降。免疫组织化学分析显示，接受治疗的受试者在术后36小时COX-2表达较高，术后第3天和第7天VEGF表达增加。我们的研究结果表明，LLLT可能通过在骨愈合初期调节炎症和血管生成基因表达以及COX2和VEGF免疫表达，有效地加速了新形成骨的发育。
