El-Halwagy Mai O, Hegazy Enas M, Shalaby Hany K, Mahmoud Elham F
Oral Biology Department, Faculty of Dentistry, Suez University, P.O.Box:43221, Suez, Egypt.
Oral Biology Department, Faculty of Dentistry, Suez Canal University, P.O.Box:41523, Ismailia, Egypt.
Lasers Med Sci. 2025 Jan 3;40(1):5. doi: 10.1007/s10103-024-04246-1.
This study aims to investigate and compare the effects of short and long-term application of low-level laser therapy on the mandibular alveolar process of osteoporotic rats. Forty adult male albino rats were included in this study. After animal grouping, the experimental group received dexamethasone (0.1 mg/kg b.wt./day for 60 days) for the induction of osteoporosis, then the rats were treated using LLLT (830 nm, 100 mW, at 60 J/cm). The lower jaw specimens were collected and processed for histological, molecular, and histomorphometric assessments. The osteoporotic group exhibited alveolar bone resorption, accompanied by significantly upregulated RANKL and downregulated OPG mRNA expression. The short-term application of laser group showed alveolar bone partial improvement with slightly downregulated RANKL and slightly upregulated OPG levels. The long-term application of laser group showed dramatic positive changes in the alveolar bone, with markedly downregulated RANKL and upregulated OPG levels. LLLT shows potential as a low-risk and impactful local management for osteoporosis, with long-term laser application demonstrably improving bone quality, quantity, and organization compared to short-term application.
本研究旨在调查和比较短期和长期应用低强度激光疗法对骨质疏松大鼠下颌牙槽突的影响。本研究纳入了40只成年雄性白化大鼠。动物分组后，实验组接受地塞米松（0.1 mg/kg体重/天，持续60天）以诱导骨质疏松，然后使用低强度激光疗法（830 nm，100 mW，60 J/cm）对大鼠进行治疗。收集下颌骨标本并进行组织学、分子学和组织形态计量学评估。骨质疏松组表现出牙槽骨吸收，同时RANKL mRNA表达显著上调，OPG mRNA表达下调。短期激光应用组显示牙槽骨部分改善，RANKL水平略有下调，OPG水平略有上调。长期激光应用组显示牙槽骨有显著的积极变化，RANKL水平显著下调，OPG水平上调。与短期应用相比，低强度激光疗法显示出作为一种低风险且有效的骨质疏松局部治疗方法的潜力，长期激光应用可显著改善骨质量、骨量和骨组织结构。
