Li Ruihua, Jiang Xiaotong, Jin Donghao, Dhaubhadel Sangeeta, Bian Shaomin, Li Xuyan
College of Plant Science, Jilin University, Changchun, China.
Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, Canada.
PLoS One. 2015 Nov 23;10(11):e0143280. doi: 10.1371/journal.pone.0143280. eCollection 2015.
14-3-3s are a class of conserved regulatory proteins ubiquitously found in eukaryotes, which play important roles in a variety of cellular processes including response to diverse stresses. Although much has been learned about 14-3-3s in several plant species, it remains unknown in common bean. In this study, 9 common bean 14-3-3s (PvGF14s) were identified by exhaustive data mining against the publicly available common bean genomic database. A phylogenetic analysis revealed that each predicted PvGF14 was clustered with two GmSGF14 paralogs from soybean. Both epsilon-like and non-epsilon classes of PvGF14s were found in common bean, and the PvGF14s belonging to each class exhibited similar gene structure. Among 9 PvGF14s, only 8 are transcribed in common bean. Expression patterns of PvGF14s varied depending on tissue type, developmental stage and exposure of plants to stress. A protein-protein interaction study revealed that PvGF14a forms dimer with itself and with other PvGF14 isoforms. This study provides a first comprehensive look at common bean 14-3-3 proteins, a family of proteins with diverse functions in many cellular processes, especially in response to stresses.
14-3-3蛋白是一类在真核生物中普遍存在的保守调节蛋白，在包括对各种胁迫的响应在内的多种细胞过程中发挥重要作用。尽管在几种植物物种中已经对14-3-3蛋白有了很多了解，但在菜豆中仍不清楚。在本研究中，通过对公开可用的菜豆基因组数据库进行详尽的数据挖掘，鉴定出了9个菜豆14-3-3蛋白（PvGF14s）。系统发育分析表明，每个预测的PvGF14都与来自大豆的两个GmSGF14旁系同源物聚类。在菜豆中发现了epsilon样和非epsilon类的PvGF14s，并且属于每个类别的PvGF14s表现出相似的基因结构。在9个PvGF14s中，菜豆中只有8个被转录。PvGF14s的表达模式因组织类型、发育阶段以及植物对胁迫的暴露情况而异。一项蛋白质-蛋白质相互作用研究表明，PvGF14a与自身以及其他PvGF14异构体形成二聚体。本研究首次全面审视了菜豆14-3-3蛋白，这是一类在许多细胞过程中具有多种功能，尤其是在响应胁迫方面具有多种功能的蛋白质家族。
