Mamuris Z, Prieur M, Dutrillaux B, Aurias A
CNRS URA 620-Structure et Mutagenèse Chromosomiques, Institut Curie, Paris, France.
Mutagenesis. 1989 Mar;4(2):133-9. doi: 10.1093/mutage/4.2.133.
The clastogenic effect of melphalan, an alkylating agent frequently used in chemotherapy, was investigated using chromosomes from human lymphocytes, two and three cell cycles after treatment in vitro. chromosome aberrations were much more frequent than chromatid type anomalies. Unbalanced rearrangements, i.e. deletions, dicentrics and complex rearrangements (in decreasing order of occurrence), were quite frequent and balanced rearrangements, such as reciprocal translocations and inversions, were quite rare. Deletions principally affected chromosomes 9, 5, 7 and 11. By comparison to the results obtained at first division after treatment, the relative frequencies of del(5) and del(20) increased with the number of cell divisions. Thus, these deletions were poorly eliminated by selection. This finding may be related to the fact that del(5) and del(20) are frequently observed in premalignant haemopathies.
使用人淋巴细胞染色体，在体外处理后的两个和三个细胞周期，对化疗中常用的烷化剂美法仑的致断裂效应进行了研究。染色体畸变比染色单体型异常更为频繁。不平衡重排，即缺失、双着丝粒和复杂重排（按发生频率递减顺序）相当常见，而平衡重排，如相互易位和倒位则相当罕见。缺失主要影响9号、5号、7号和11号染色体。与处理后第一次分裂时获得的结果相比，del(5)和del(20)的相对频率随细胞分裂次数增加。因此，这些缺失通过选择很难消除。这一发现可能与在癌前血液病中经常观察到del(5)和del(20)这一事实有关。
