Mamuris Z, Gerbault-Seureau M, Prieur M, Pouillart P, Dutrillaux B, Aurias A
C.N.R.S. UA 620 Structure et Mutagenèse Chromosomiques, Institut Curie, Paris, France.
Int J Cancer. 1989 Jan 15;43(1):80-6. doi: 10.1002/ijc.2910430117.
Chromosome lesions detected in lymphocytes from 14 patients previously treated with melphalan, a bifunctional alkylating agent, have been analyzed on R-banded preparations. In comparison to controls, there was no significant increase of chromatid-type lesions, but chromosome-type lesions were quite frequent, affecting 21.5% of metaphases, on the average. Reciprocal translocations represent 54%, unbalanced translocations 15%, deletions 19% and inversions 6% of all rearrangements. Most of these would not have been detected without the use of chromosome banding. The distributions of affected chromosomes and chromosome bands were not random. Almost all imbalances resulting from rearrangements lead to losses but not to gains. The distribution of the abnormal chromosomes has been compared to that observed in controls and in in vitro experiments, and to the characteristic pattern of malignant cells from patients affected by secondary acute leukemia (ANLL).
对14名曾接受双功能烷化剂美法仑治疗的患者淋巴细胞中检测到的染色体损伤，已在R带制备物上进行了分析。与对照组相比，染色单体型损伤没有显著增加，但染色体型损伤相当常见，平均影响21.5%的中期细胞。相互易位占所有重排的54%，不平衡易位占15%，缺失占19%，倒位占6%。如果不使用染色体显带技术，其中大多数损伤将无法被检测到。受影响的染色体和染色体带的分布并非随机。重排导致的几乎所有不平衡都导致缺失而非增加。已将异常染色体的分布与对照组、体外实验中观察到的分布以及继发性急性白血病（ANLL）患者恶性细胞的特征模式进行了比较。
