Forcucci Alessandra, Pawlowski Michal E, Majors Catherine, Richards-Kortum Rebecca, Tkaczyk Tomasz S
Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77030, USA.
Biomed Opt Express. 2015 Oct 19;6(11):4433-46. doi: 10.1364/BOE.6.004433. eCollection 2015 Nov 1.
Three-part differential white blood cell counts are used for disease diagnosis and monitoring at the point-of-care. A low-cost, miniature achromatic microscope was fabricated for identification of lymphocytes, monocytes, and granulocytes in samples of whole blood stained with acridine orange. The microscope was manufactured using rapid prototyping techniques of diamond turning and 3D printing and is intended for use at the point-of-care in low-resource settings. The custom-designed microscope requires no manual adjustment between samples and was successfully able to classify three white blood cell types (lymphocytes, granulocytes, and monocytes) using samples of peripheral whole blood stained with acridine orange.
三分群白细胞计数用于即时医疗中的疾病诊断和监测。制造了一种低成本的微型消色差显微镜，用于识别用吖啶橙染色的全血样本中的淋巴细胞、单核细胞和粒细胞。该显微镜采用金刚石车削和3D打印的快速成型技术制造，旨在用于资源匮乏地区的即时医疗。定制设计的显微镜在样本之间无需手动调整，并且使用用吖啶橙染色的外周全血样本成功地对三种白细胞类型（淋巴细胞、粒细胞和单核细胞）进行了分类。
