Zhu Xiao-min, Huang Yi-min, Fan Jian-feng, Shi Ying-zuo
Pharmazie. 2015 Oct;70(10):656-60.
Regulatory B cells (Breg) are a distinct B cell subset, which contribute to the pathogenesis of autoimmune disorders. Interleukin-10 (IL-10) plays a pivotal function to Breg. It is well described in adults but little is known in a pediatric population. This study was to investigate the role of IL-10-producing B cell (B10) and its association with Treg and Th17 subsets in the children with inflammatory bowel diseases (IBD). Peripheral blood mononuclear cells from IBD children patients and controls were stimulated with PMA, ionomycin, and brefeldin A. The frequencies of CD19+IL-10+ B cells, CD3+CD4+IL-17+Th17 cells, and CD4+ CD25(hi)Foxp3+ Treg cells were analyzed by flow cytometry. The mRNA expression of Foxp3, IL-17a and RORγt was detected by real-time quantitative PCR. The number of B10 cells was elevated in IBD children patients. There was a positive correlation between B10 cells and Tregs in IBD. The ratio of Treg/Th17 decreased in IBD, and it strongly correlated with B10 cells. The frequency of B10 cells is elevated in IBD and it correlates with both the Tregs counts and the Treg/Th17 ratio. B10 cells to regulate functional T cell subsets might be impaired in paediatric patients with IBD.
调节性B细胞（Breg）是一种独特的B细胞亚群，其与自身免疫性疾病的发病机制有关。白细胞介素-10（IL-10）对Breg发挥关键作用。其在成人中已有充分描述，但在儿科人群中知之甚少。本研究旨在探讨产生IL-10的B细胞（B10）在儿童炎症性肠病（IBD）中的作用及其与调节性T细胞（Treg）和辅助性T细胞17（Th17）亚群的关系。用佛波酯、离子霉素和布雷菲德菌素A刺激IBD患儿和对照的外周血单个核细胞。通过流式细胞术分析CD19 + IL-10 + B细胞、CD3 + CD4 + IL-17 + Th17细胞和CD4 + CD25（高）Foxp3 + Treg细胞的频率。通过实时定量PCR检测Foxp3、IL-17a和维甲酸相关孤儿受体γt（RORγt）的mRNA表达。IBD患儿中B10细胞数量升高。IBD中B10细胞与Treg之间呈正相关。IBD中Treg/Th17比值降低，且与B10细胞密切相关。IBD中B10细胞频率升高，且与Treg计数和Treg/Th17比值均相关。IBD患儿中调节功能性T细胞亚群的B10细胞可能受损。
