Zhu Xiao-Min, Shi Ying-Zuo, Cheng Ming, Wang Da-Feng, Fan Jian-Feng
Pharmazie. 2017 May 1;72(5):283-287. doi: 10.1691/ph.2017.6957.
IL-6 and IL-23 are both pleiotropic cytokines involved in the regulation of the immune response, inflammation, and hematopoeisis. They also could mediate effector cells and tolerance mediated by cells with regulatory function. Inflammatory bowel disease (IBD) is associated with a reduced ratio of Treg cells ato Th17 effector cells in peripheral blood and is characterised by a pro-inflammatory cytokine microenvironment which supports the continued generation of Th17 cells. It is well described in adults but little is known in a pediatric population. This study was aimed to investigate the role of IL-6, IL-23 and its association with Treg and Th17 subsets in pediatric IBD patients. Peripheral blood mononuclear cells from patients and controls were stimulated with PMA, ionomycin, and brefeldin A. The frequencies of CD4+Foxp3+ cells, and CD4+IL17a+ cells were analyzed by flow cytometry. The serum level of IL-6 and IL-23 was determined by Elisa kit. The mRNA expression of Foxp3, IL-17a, IL-6 and IL-23 was detected by real-time quantitative PCR. The ratio of Treg/Th17 decreased in pediatric IBD patients, and it strongly correlated with IL-6 and IL-23. The present study provides a quantitative analysis regarding the Th17/Treg cell balance in peripheral blood of children with IBD and its association with serum IL-6 and IL-23 level.
白细胞介素-6（IL-6）和白细胞介素-23（IL-23）都是多效性细胞因子，参与免疫反应、炎症和造血的调节。它们还可介导具有调节功能的细胞所介导的效应细胞和耐受性。炎症性肠病（IBD）与外周血中调节性T细胞（Treg）与Th17效应细胞的比例降低有关，其特征是促炎细胞因子微环境支持Th17细胞的持续产生。这在成人中已有充分描述，但在儿科人群中了解甚少。本研究旨在探讨IL-6、IL-23在儿科IBD患者中的作用及其与Treg和Th17亚群的关系。用佛波酯（PMA）、离子霉素和布雷菲德菌素A刺激患者和对照组的外周血单个核细胞。通过流式细胞术分析CD4+Foxp3+细胞和CD4+IL17a+细胞的频率。用酶联免疫吸附测定（ELISA）试剂盒测定血清IL-6和IL-23水平。通过实时定量聚合酶链反应（PCR）检测Foxp3、IL-17a、IL-6和IL-23的mRNA表达。儿科IBD患者的Treg/Th17比值降低，且与IL-6和IL-23密切相关。本研究提供了关于IBD患儿外周血中Th17/Treg细胞平衡及其与血清IL-6和IL-23水平关系的定量分析。
