Chemical Characterization and Wound Healing Property of Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms.

BACKGROUND

Cham., known as carobinha, is prevalent in the Cerrado biome and presents popular use in treatment of dermatological diseases. The present study aimed to investigate the healing action of topical formulation of Cham. (FtEHJ) in mice cutaneous lesions.

METHODS

Phytochemical analysis of hydroalcoholic extract was carried out by using HPLC-PDA-ESI-MS and FIA-ESI-IT-MSn. Swiss mice were treated topically with formulation base (FtB) or Fibrinase® or ointment FtEHJ (15 mg/g; 50 mg/Kg). At the end of treatment periods, the inflammatory cytokines (TNF-, IL-1 and IL-6) in the lesions were measured by using ELISA and gene expression of TGF-, Collagen I, and Collagen III was demonstrated by RTqPCR method and histological evaluation.

RESULTS

Ten compounds were identified in the extract, distributed among the classes of flavonoids and triterpenes. Treatment with FtEHJ increased the wound contraction in 24 hours, such as reduction of TNF-, IL-1, and IL-6 (pg/mL) cytokines in the lesion. The TGF- and collagen gene expression was increased and the wound closure accelerated to nine days, with discrete inflammation, collagenization, and accented reepithelialization. Conclusions. The results obtained suggest chemical compounds present in the FtEHJ accelerates wound healing by being a gene expression modulator, and protein content of different molecules are involved in tissue repair.