Sun Yaning, Xing Guangxu, Yang Jifei, Wang Fangyu, Deng Ruiguang, Zhang Gaiping, Hu Xiaofei, Zhang Yong
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China.
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
J Sci Food Agric. 2016 Aug;96(11):3673-8. doi: 10.1002/jsfa.7550. Epub 2015 Dec 29.
Ochratoxin A (OTA) and zearalenone (ZEN) are natural products of filamentous fungi that are harmful to humans and animals exposed to them even in extremely low concentration. The immunochromatographic test strip has become a popular diagnostic tool for detecting analytes. Its major advantages are that results can be obtained within 5-10 min, all needed reagents are included in the strip and it can be used to detect OTA and ZEN contamination in spots. In this study a colloidal gold-based immunochromatographic test strip of competitive format was developed for the rapid simultaneous qualitative and quantitative detection of OTA and ZEN in corn and other cereals.
The test strip results with the naked eye showed that the sensitivities were 6 µg kg(-1) OTA and 20 µg kg(-1) ZEN in cereal, while those with a TSR3000 membrane strip reader showed that the IC50 values of OTA and ZEN were 1.7905 and 4.3514 ng mL(-1) and the lower detection limit (LDL) values were 0.7697 and 1.2000 µg kg(-1) respectively. These results were confirmed by high-performance liquid chromatography.
The immunochromatographic test strip developed in this study could be used for the rapid simultaneous, qualitative and quantitative screening of OTA and ZEN in corn samples. © 2015 Society of Chemical Industry.
赭曲霉毒素A（OTA）和玉米赤霉烯酮（ZEN）是丝状真菌的天然产物，即使在极低浓度下，接触到它们的人类和动物也会受到伤害。免疫层析试纸条已成为检测分析物的一种流行诊断工具。其主要优点是可在5 - 10分钟内获得结果，试纸条中包含所有所需试剂，并且可用于现场检测OTA和ZEN污染。在本研究中，开发了一种基于胶体金的竞争型免疫层析试纸条，用于快速同时定性和定量检测玉米及其他谷物中的OTA和ZEN。
肉眼观察试纸条结果显示，谷物中OTA和ZEN的灵敏度分别为6 μg kg⁻¹和20 μg kg⁻¹，而使用TSR3000膜条读数仪的结果显示，OTA和ZEN的半数抑制浓度（IC50）值分别为1.7905和4.3514 ng mL⁻¹，最低检测限（LDL）值分别为0.7697和1.2000 μg kg⁻¹。这些结果通过高效液相色谱法得到了证实。
本研究开发的免疫层析试纸条可用于玉米样品中OTA和ZEN的快速同时定性和定量筛查。© 2015化学工业协会。
