School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
National & Local United Engineering Lab of Rapid Diagnostic Test, Guangzhou Wondfo Biotech Co., Ltd., Guangzhou 510663, China.
Toxins (Basel). 2020 Nov 19;12(11):722. doi: 10.3390/toxins12110722.
colitis is caused by a cytotoxin produced by the anaerobic bacteria in the epithelial cells of the large intestine, particularly toxin B (TcdB). However, the sensitivity of currently utilized endotoxin determination methods has been called into question, and, therefore, more accurate and convenient detection methods are needed. Our study is the first to systematically compare fluorescent submicrosphere-based and quantum-dot nanobead-based lateral fluidity measurement methods (FMs-LFA and QDNBs-LFA) with toxin B quantification in fecal samples via sandwich analysis. The limits of detection (LOD) of FMs-LFA and QDNBs-LFA in the fecal samples were 0.483 and 0.297 ng/mL, respectively. TcdB analyses of the fecal samples indicated that the results of QDNBs-LFA and FMs-LFA were consistent with those of a commercial enzyme-linked immunosorbent assay (ELISA) test kit. The sensitivity of QDNBs-LFA was highly correlated with clinical diagnoses. Therefore, quantum dot nanobeads (QDNBs) are deemed highly suitable for lateral fluidity analyses, which would facilitate the implementation of portable and rapid on-the-spot applications, such as food hygiene and safety tests and onsite medical testing.
结肠炎是由大肠上皮细胞中的厌氧细菌产生的细胞毒素引起的，特别是毒素 B（TcdB）。然而，目前使用的内毒素测定方法的灵敏度受到质疑，因此需要更准确和方便的检测方法。我们的研究首次通过夹心分析系统比较了基于荧光亚微米球和量子点纳米珠的横向流动性测量方法（FMs-LFA 和 QDNBs-LFA）与粪便样本中 TcdB 定量的关系。FMs-LFA 和 QDNBs-LFA 在粪便样本中的检测限（LOD）分别为 0.483 和 0.297 ng/mL。粪便样本的 TcdB 分析表明，QDNBs-LFA 和 FMs-LFA 的结果与商业酶联免疫吸附测定（ELISA）试剂盒的结果一致。QDNBs-LFA 的灵敏度与临床诊断高度相关。因此，量子点纳米珠（QDNBs）非常适合用于横向流动性分析，这将有助于实现便携式和快速现场应用，如食品卫生和安全测试以及现场医疗测试。
