Sołtys-Kalina Dorota, Szajko Katarzyna, Sierocka Izabela, Śliwka Jadwiga, Strzelczyk-Żyta Danuta, Wasilewicz-Flis Iwona, Jakuczun Henryka, Szweykowska-Kulinska Zofia, Marczewski Waldemar
Plant Breeding and Acclimatization Institute, National Research Institute, Młochów, Platanowa 19, 05-831 Młochów, Poland.
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Mol Breed. 2015;35(12):224. doi: 10.1007/s11032-015-0415-1. Epub 2015 Nov 18.
Potato ( L.) tubers exhibit significant variation in reducing sugar content directly after harvest, cold storage and reconditioning. Here, we performed QTL analysis for chip color, which is strongly influenced by reducing sugar content, in a diploid potato mapping population. Two QTL on chromosomes I and VI were detected for chip color after harvest and reconditioning. Only one region on chromosome VI was linked with cold-induced sweetening. Using the RT-PCR technique, we showed differential expression of the - () gene. The transcript was presented in light chip color parental clone DG 97-952 and the RNA progeny of the bulk sample consisting of light chip color phenotypes after cold storage. This amplicon was absent in dark chip parental clone DG 08-26/39 and the RNA bulk sample of dark chip progeny. Genetic variation of explained up to 16.6 and 15.2 % of the phenotypic variance after harvest and 3 months of storage at 4 °C, respectively. Using an alternative approach, the RDA-cDNA method was used to recognize 25 gene sequences, of which 11 could be assigned to potato chromosome VI. One of these genes, (), demonstrated higher mRNA and protein expression in RT-qPCR and western blotting assays in the dark chip color progeny bulk sample compared with the light chip color progeny bulk sample. Our study, for the first time, suggests that the and genes are novel candidate genes capable of influencing the chip color of potato tubers.
马铃薯（L.）块茎在收获后、冷藏和回温处理后，还原糖含量存在显著差异。在此，我们在一个二倍体马铃薯作图群体中，对受还原糖含量强烈影响的薯片颜色进行了QTL分析。在收获和回温处理后，在第I和第VI号染色体上检测到两个与薯片颜色相关的QTL。只有第VI号染色体上的一个区域与冷诱导甜化有关。利用RT-PCR技术，我们展示了-（）基因的差异表达。该转录本存在于浅薯片颜色亲本克隆DG 97-952以及冷藏后由浅薯片颜色表型组成的混合样本的RNA后代中。在深薯片颜色亲本克隆DG 08-26/39和深薯片后代的RNA混合样本中不存在该扩增子。分别在收获后和4℃储存3个月后，的遗传变异分别解释了高达16.6%和15.2%的表型变异。使用另一种方法，RDA-cDNA方法用于识别25个基因序列，其中11个可定位到马铃薯第VI号染色体。这些基因中的一个，（），在RT-qPCR和蛋白质免疫印迹分析中，与浅薯片颜色后代混合样本相比，在深薯片颜色后代混合样本中表现出更高的mRNA和蛋白质表达。我们的研究首次表明，和基因是能够影响马铃薯块茎薯片颜色的新候选基因。
