State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Xianyang, 712100, Shaanxi, China.
Mol Biol Rep. 2020 Sep;47(9):6679-6691. doi: 10.1007/s11033-020-05722-x. Epub 2020 Aug 11.
Heat shock protein 90 genes/proteins (Hsp90s) are related to the stress resistance found in various plant species. These proteins affect the growth and development of plants and have important effects on the plants under various stresses (cold, drought and salt) in the environment. In this study, we identified 334 Hsp90s from 43 plant species, and Hsp90s were found in all species. Phylogenetic tree and conserved domain database analysis of all Hsp90s showed three independent clades. The analysis of motifs, gene duplication events, and the expression data from PGSC website revealed the gene structures, evolution relationships, and expression patterns of the Hsp90s. In addition, analysis of the transcript levels of the 7 Hsp90s in potato (Solanum tuberosum) under low temperature and high temperature stresses showed that these genes were related to the temperature stresses. Especially StHsp90.2 and StHsp90.4, under high or low temperature conditions, the expression levels in leaves, stems, or roots were significantly up-regulated. Our findings revealed the evolution of the Hsp90s, which had guiding significance for further researching the precise functions of the Hsp90s.
热休克蛋白 90 基因/蛋白（Hsp90s）与各种植物物种的抗逆性有关。这些蛋白质影响植物的生长和发育，对环境中各种胁迫（冷、旱、盐）下的植物有重要影响。在这项研究中，我们从 43 种植物中鉴定出 334 种 Hsp90，所有物种都存在 Hsp90。对所有 Hsp90 的系统发育树和保守结构域数据库分析表明存在三个独立的分支。基序分析、基因复制事件以及 PGSC 网站的表达数据揭示了 Hsp90 的基因结构、进化关系和表达模式。此外，对马铃薯（Solanum tuberosum）在低温和高温胁迫下的 7 个 Hsp90 转录水平的分析表明，这些基因与温度胁迫有关。特别是 StHsp90.2 和 StHsp90.4，在高低温条件下，叶片、茎或根中的表达水平显著上调。我们的研究结果揭示了 Hsp90 的进化，这对进一步研究 Hsp90 的精确功能具有指导意义。
