Kumar Sandeep, Lather Viney, Pandita Deepti
Department of Pharmaceutics, JCDM College of Pharmacy, Sirsa 125055, Haryana, India.
Department of Pharmaceutical Chemistry, JCDM College of Pharmacy, Sirsa 125055, Haryana, India.
Food Chem. 2016 Apr 15;197(Pt A):959-64. doi: 10.1016/j.foodchem.2015.11.078. Epub 2015 Nov 21.
Resveratrol and quercetin are well-known polyphenolic compounds present in common foods, which have demonstrated enormous potential in the treatment of a wide variety of diseases. Owing to their exciting synergistic potential and combination delivery applications, we developed a simple and rapid RP-HPLC method based on isosbestic point detection. The separation was carried out on phenomenex Synergi 4μ Hydro-RP 80A column using methanol: acetonitrile (ACN): 0.1% phosphoric acid (60:10:30) as mobile phase. The method was able to quantify nanograms of analytes simultaneously on a single wavelength (269 nm), making it highly sensitive, rapid as well as economical. Additionally, forced degradation studies of resveratrol and quercetin were established and the method's applicability was evaluated on PLGA nanoparticles and human plasma. The analytes peaks were found to be well resolved in the presence of degradation products and excipients. The simplicity of the developed method potentializes its suitability for routine in vitro and in vivo analysis of resveratrol and quercetin.
白藜芦醇和槲皮素是常见食物中存在的著名多酚类化合物，它们在治疗多种疾病方面已显示出巨大潜力。由于它们令人兴奋的协同潜力和联合递送应用，我们基于等吸收点检测开发了一种简单快速的反相高效液相色谱（RP-HPLC）方法。分离在菲罗门Synergi 4μ Hydro-RP 80A柱上进行，使用甲醇：乙腈（ACN）：0.1%磷酸（60:10:30）作为流动相。该方法能够在单一波长（269 nm）下同时定量分析物的纳克量，使其具有高灵敏度、快速且经济的特点。此外，建立了白藜芦醇和槲皮素的强制降解研究，并在聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒和人血浆上评估了该方法的适用性。发现在存在降解产物和辅料的情况下，分析物峰得到了很好的分离。所开发方法的简便性使其适用于白藜芦醇和槲皮素的常规体外和体内分析。
