Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba , 196 Innovation Drive, Winnipeg, Manitoba R3T 2N2, Canada.
J Agric Food Chem. 2014 Jun 25;62(25):5812-7. doi: 10.1021/jf5001277. Epub 2014 Jun 17.
The objectives of the study were to develop a reverse-phase high-performance liquid chromatography method for the simultaneous analyses of trans-/cis-resveratrol, emodin, and quercetin and to determine the concentrations of these polyphenols in 28 resveratrol supplements. Samples were separated within 15 min in a C18 reversed-phase column using mobile phases containing 0.1% formic acid and methanol/0.1% formic acid. The calibration graphs for all four compounds were linear from 0.1 to 410 μg/mL (r2=0.99). The concentration of resveratrol as stated on the labels was often different from the analytical results, with 21 and 11% of the total supplements having low or high values, respectively. Many of the supplements also contained variable but generally low levels of emodin, a compound known to cause diarrhea. The proposed method is a rapid, sensitive, accurate, and cost-effective procedure that can be used for the simultaneous quantification of four polyphenols in resveratrol supplements.
本研究的目的是开发一种反相高效液相色谱法，用于同时分析反式/顺式白藜芦醇、大黄素和槲皮素，并测定 28 种白藜芦醇补充剂中这些多酚的浓度。样品在 C18 反相柱中，在含有 0.1%甲酸和甲醇/0.1%甲酸的流动相作用下，15 分钟内完成分离。所有四种化合物的校准曲线在 0.1 到 410 μg/mL 范围内呈线性（r2=0.99）。标签上标注的白藜芦醇浓度往往与分析结果不同，分别有 21%和 11%的总补充剂存在低值或高值。许多补充剂还含有不同但通常较低水平的大黄素，这种化合物已知会引起腹泻。该方法快速、灵敏、准确且具有成本效益，可用于同时定量测定白藜芦醇补充剂中的四种多酚。
