Moretón-Lamas E, Lago-Crespo M, Lage-Yusty M A, López-Hernández J
University of Santiago de Compostela, Faculty of Pharmacy, Department of Analytical Chemistry, Nutrition and Bromatology, Campus Vida, 15782 Santiago de Compostela, Spain.
University of Santiago de Compostela, Faculty of Pharmacy, Department of Analytical Chemistry, Nutrition and Bromatology, Campus Vida, 15782 Santiago de Compostela, Spain.
Food Chem. 2017 Jun 1;224:219-223. doi: 10.1016/j.foodchem.2016.12.060. Epub 2016 Dec 21.
Resveratrol is a stilbene, which is one of a group of polyphenols in many plant foods. Interest in this substance lies in its potential health benefits. This study aimed to compare two different methods, chromatographic and constant-wavelength synchronous spectrofluorimetry, an alternative technique to determine the amount of resveratrol dietary supplements, as a model for more complex foods. High-performance liquid chromatography-photodyode array detector-mass spectrometry/mass spectrometry was used to confirm the results. The results obtained showed that both methods were valid for the determination of resveratrol in dietary supplements. HPLC with fluorescence and variable wavelength detectors offered better linearity and sensitivity, and would be more suitable for the determination of several stilbenes in complex samples. On the other hand, constant-wavelength synchronous spectrofluorimetry is a sensitive, rapid and inexpensive method that could be used for quick and precise determination when samples are expected to contain only one stilbene.
白藜芦醇是一种芪类化合物，是许多植物性食物中多酚类物质的一种。人们对这种物质感兴趣是因其潜在的健康益处。本研究旨在比较两种不同的方法，即色谱法和等波长同步荧光光谱法，后者是一种用于测定白藜芦醇膳食补充剂含量的替代技术，以此作为更复杂食物的模型。采用高效液相色谱 - 光电二极管阵列检测器 - 质谱/质谱联用技术来验证结果。所得结果表明，两种方法对于测定膳食补充剂中的白藜芦醇均有效。配备荧光和可变波长检测器的高效液相色谱法具有更好的线性和灵敏度，更适合于测定复杂样品中的几种芪类化合物。另一方面，等波长同步荧光光谱法是一种灵敏、快速且廉价的方法，当预期样品仅含有一种芪类化合物时，可用于快速且精确的测定。
