Miertus S, Katrlík J, Pizzariello A, Stred'anský M, Svitel J, Svorc J
International Centre for Science and High Technology, UNIDO Area/Science Park, Trieste, Italy.
Biosens Bioelectron. 1998 Oct 1;13(7-8):911-23. doi: 10.1016/s0956-5663(98)00063-3.
Solid binding matrix (SBM) based composite transducers have been used for development of series of multibiosensor systems applicable in various fields. Here we present two hybrid three-channel multibiosensors for simultaneous amperometric operation in food quality control, i.e. glucose/fructose/ethanol multibiosensor, based on glucose oxidase/fructose dehydrogenase/alcohol dehydrogenase surface-modified enzyme electrodes and L-lactate/L-malate/sulfite multibiosensor, based on L-lactate dehydrogenase/L-malate dehydrogenase/sulfite oxidase surface-modified enzyme electrodes. Different parameters have been studied in order to optimize the response of the multibiosensor systems. The multibiosensor showed a good sensitivity, linear range and storage stability. The multibiosensors were used for the determination of glucose, fructose, ethanol, L-lactate, L-malate and sulfite in samples of wine, resulting in a good agreement with data certified by the supplier. Comparison of various designs, surface-modified, bulk-modified and thick-cover, of SBM based biosensors is studied on the example of fructose biosensor.
基于固体结合基质（SBM）的复合换能器已被用于开发一系列适用于各个领域的多生物传感器系统。在此，我们展示了两种用于食品质量控制中同时进行安培检测操作的混合三通道多生物传感器，即基于葡萄糖氧化酶/果糖脱氢酶/乙醇脱氢酶表面修饰酶电极的葡萄糖/果糖/乙醇多生物传感器，以及基于L - 乳酸脱氢酶/L - 苹果酸脱氢酶/亚硫酸盐氧化酶表面修饰酶电极的L - 乳酸/L - 苹果酸/亚硫酸盐多生物传感器。为了优化多生物传感器系统的响应，对不同参数进行了研究。该多生物传感器表现出良好的灵敏度、线性范围和储存稳定性。这些多生物传感器用于测定葡萄酒样品中的葡萄糖、果糖、乙醇、L - 乳酸、L - 苹果酸和亚硫酸盐，结果与供应商认证的数据高度吻合。以果糖生物传感器为例，研究了基于SBM的生物传感器的各种设计（表面修饰、本体修饰和厚覆盖）之间的比较。
