Piperno G, Huang B, Luck D J
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1600-4. doi: 10.1073/pnas.74.4.1600.
Flagellar polypeptides of Chlamydomonas reinhardtii were analyzed in two-dimensions by isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate. In addition to flagellar tubulin, over 130 polypeptides were resolved and 100 of these were identified as axonemal components in wild-type organisms. Flagella of two nonconditional paralyzed mutants, pf 14 and pf 1, were also analyzed and, at the same time, electron microscopic studies were carried out. pf 14 flagella, which completely lack radial spokes and associated spokeheads, are missing 12 polypeptides. Six of these polypeptides are also missing from pf 1 flagella in which spokes are clearly present but spoke heads appear to be absent.
莱茵衣藻的鞭毛多肽通过等电聚焦和在十二烷基硫酸钠存在下的电泳进行二维分析。除了鞭毛微管蛋白外，还分离出了130多种多肽，其中100种被鉴定为野生型生物体中的轴丝成分。还分析了两个非条件性麻痹突变体pf 14和pf 1的鞭毛，同时进行了电子显微镜研究。完全缺乏径向辐条和相关辐条头的pf 14鞭毛缺少12种多肽。其中六种多肽在pf 1鞭毛中也不存在，pf 1鞭毛中辐条明显存在，但辐条头似乎不存在。
