Sharbatkhori Mitra, Nazemalhosseini Mojarad Ehsan, Taghipour Niloofar, Pagheh Abdol Sattar, Mesgarian Fatemeh
Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran ; Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2015 Jul-Sep;10(3):441-7.
Cryptosporidium is an intestinal protozean parasite causing waterborne and foodborne outbreaks of diarrheal diseases. The present study was performed in order to find prevalence and subtypes of Cryptosporidium among children with diarrhea in Gonbad Kavoos City, Northern Iran.
Diarrheic samples were collected from 547 children. The initial parasitological diagnosis was made based on detection of oocysts using the modified Ziehl-Neelsen acid-fast staining method. The positive microscopically samples were selected for sequence analysis of partial 60 kDa glycoprotein (gp60) gene.
Out of 547 collected samples, 27 (4.94%) were positive for Cryptosporidium oocysts. Fifteen from 27 positive samples successfully amplified in PCR. Sequences analysis of gp60 gene in 15 Cryptosporidium isolates revealed that all of them (100%) were C. parvum. The results showed three subtypes of IIa subtype family (7 cases) including IIaA16G2R1, IIaA17G1R1, IIaA22G3R1 and one subtype of IId subtype family (8 cases). The most common allele was IId A17G1d (53.3%).
The predominance of zoonotic subtype families of C. parvum species (IIa, IId) in the present study is in concordance with previous studies in Iran and emphasizes the significance of zoonotic transmission of cryptosporidiosis in the country.
隐孢子虫是一种肠道原生动物寄生虫，可引发经水和食物传播的腹泻病暴发。本研究旨在查明伊朗北部贡巴德卡武斯市腹泻儿童中隐孢子虫的流行情况和亚型。
从547名儿童中采集腹泻样本。最初的寄生虫学诊断是基于使用改良齐-尼抗酸染色法检测卵囊。对显微镜检查呈阳性的样本进行部分60 kDa糖蛋白（gp60）基因的序列分析。
在547份采集的样本中，27份（4.94%）隐孢子虫卵囊呈阳性。27份阳性样本中有15份在PCR中成功扩增。对15株隐孢子虫分离株的gp60基因进行序列分析显示，所有分离株（100%）均为微小隐孢子虫。结果显示IIa亚型家族有三种亚型（7例），包括IIaA16G2R1、IIaA17G1R1、IIaA22G3R1，IId亚型家族有一种亚型（8例）。最常见的等位基因是IId A17G1d（53.3%）。
本研究中微小隐孢子虫物种的人畜共患亚型家族（IIa、IId）占优势，这与伊朗此前的研究结果一致，并强调了该国隐孢子虫病人畜共患传播的重要性。
