Meng Zhuangzhi, Li Jianguo, Zhao Honglin, Liu Haiying, Zhang Guowei, Wang Lingzhan, Hu H E, Li D I, Liu Mingjing, Bi Fulong, Wang Xiaoping, Tian Geng, Liu Qiang, Buren Batu
Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China.
Department of Human Anatomy, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Department of Preventive Medicine, The School of Medicine of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028041, P.R. China ; Laboratory of Biomedicine and Department of Mongolian Medicine Hematology-Oncology, The Affiliated Hospital of Inner Mongolia University for The Nationalities, Tongliao, Inner Mongolia 028007, P.R. China.
Exp Ther Med. 2015 Aug;10(2):525-530. doi: 10.3892/etm.2015.2555. Epub 2015 Jun 5.
Resveratrol, a naturally occurring phytoalexin, acts as an activator of sirtuin 1 (SIRT1) and has been shown to have a neuroprotective role in various models. Healthy adult male Sprague-Dawley rats were subjected to cerebral ischemia in order to study the protective effect of resveratrol on the brain following ischemia, and to investigate the effects of SIRT1 activation on the hippocampus. Untreated and resveratrol-treated rats were anesthetized prior to undergoing surgery to induce middle cerebral artery occlusion followed by reperfusion. SIRT1 expression was evaluated by immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction, and SIRT1 activity was also evaluated. In addition, terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) and Nissl staining assays were conducted and the levels of reactive oxygen species were determined. It was observed that resveratrol significantly decreased the number of TUNEL-positive cells and increased the expression of SIRT1 mRNA in a dose-dependent manner. This was accompanied by increases in SIRT1 protein expression levels and SIRT1 activity. The results demonstrate the neuroprotective and antioxidant effects of resveratrol against ischemia-induced apoptosis in the rat hippocampus.
白藜芦醇是一种天然存在的植物抗毒素，作为沉默信息调节因子1（SIRT1）的激活剂，已在多种模型中显示出具有神经保护作用。为了研究白藜芦醇对缺血后脑组织的保护作用，并探讨SIRT1激活对海马体的影响，将健康成年雄性Sprague-Dawley大鼠进行脑缺血处理。在进行诱导大脑中动脉闭塞再灌注的手术前，对未处理和经白藜芦醇处理的大鼠进行麻醉。通过免疫组织化学、蛋白质印迹法和逆转录定量聚合酶链反应评估SIRT1表达，并评估SIRT1活性。此外，进行了末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）和尼氏染色分析，并测定了活性氧水平。观察到白藜芦醇以剂量依赖性方式显著减少TUNEL阳性细胞数量，并增加SIRT1 mRNA的表达。这伴随着SIRT1蛋白表达水平和SIRT1活性的增加。结果证明了白藜芦醇对大鼠海马体缺血诱导的细胞凋亡具有神经保护和抗氧化作用。
