Shi Xiumin, Xu Jianting, Wang Jihan, Cui Meizi, Gao Yushun, Niu Haitao, Jin Haofan
Cancer Centre, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Pathogenobiology, Basic Medical College of Jilin University, Changchun, Jilin 130021, P.R. China.
Oncol Lett. 2015 Sep;10(3):1309-1314. doi: 10.3892/ol.2015.3447. Epub 2015 Jul 1.
Gastric cancer is a common type of cancer worldwide, and has a poor prognosis, in part due to the low rates of early diagnosis and the limited treatment methods available. Apolipoprotein E (ApoE) is involved in exogenous cholesterol transport and may be important in enabling tumor cells to fulfill their high cholesterol requirements. A number of reports have indicated that ApoE affects the development and prognosis of gastric cancer. Therefore, the aim of the present study was to investigate the genes and transcription factors that interact with ApoE during the development of gastric cancer. Using gene expression profiling, the BioGRID database and the transcriptional regulatory element database, gene expression and regulatory networks in gastric cancer tissues and adjacent normal tissues were analyzed. The data demonstrated that eight genes associated with ApoE were differentially expressed, with six of these upregulated and two downregulated. Functionally, these genes were involved in the JAK-STAT cascade, acute-phase response, acute inflammatory response, and the steroid hormone response. Among these ApoE-associated genes, expression of the signal transducer and activator of transcription 2 (STAT2) and STAT3 transcription factors was upregulated. To the best of our knowledge, this is the first study to demonstrate the network of ApoE-related genes and transcription factors in gastric cancer. Additional studies are required in order to confirm these data and to translate the results into the identification of clinical biomarkers and novel treatment strategies for gastric cancer.
胃癌是全球常见的癌症类型，预后较差，部分原因是早期诊断率低以及可用的治疗方法有限。载脂蛋白E（ApoE）参与外源性胆固醇转运，可能在使肿瘤细胞满足其高胆固醇需求方面发挥重要作用。多项报告表明，ApoE影响胃癌的发生发展和预后。因此，本研究的目的是探讨在胃癌发生过程中与ApoE相互作用的基因和转录因子。利用基因表达谱、BioGRID数据库和转录调控元件数据库，分析了胃癌组织和癌旁正常组织中的基因表达和调控网络。数据表明，与ApoE相关的8个基因存在差异表达，其中6个上调，2个下调。在功能上，这些基因参与JAK-STAT级联反应、急性期反应、急性炎症反应和类固醇激素反应。在这些与ApoE相关的基因中，信号转导和转录激活因子2（STAT2）和STAT3转录因子的表达上调。据我们所知，这是第一项证明胃癌中ApoE相关基因和转录因子网络的研究。需要进一步的研究来证实这些数据，并将结果转化为胃癌临床生物标志物的鉴定和新的治疗策略。
