Qu Wei, Fu Jin-Dong, Yang Fang, Yang Gong-Li, Zhang Ya-Li, Wang Xin-Ying, Gu Hong-Xiang, Zhang Hai-Yan, Wang Ling
Internal Medicine - Department of Digestive Diseases, Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China ; Internal Medicine - Department of Digestive Diseases, Rizhao People's Hospital, Rizhao, Shandong 276800, P.R. China.
Internal Medicine - Department of Digestive Diseases, Shandong Medical University, Jinan, Shandong 250012, P.R. China.
Oncol Lett. 2015 Sep;10(3):1409-1415. doi: 10.3892/ol.2015.3431. Epub 2015 Jun 29.
There are limitations to the use of single biomarker levels, for example phosphate and tensin homology (PTEN) or vascular endothelial growth factor (VEGF), in the diagnosis of esophageal squamous cell carcinoma (ESCC). The present study therefore aimed to evaluate the clinical implications of combined detection of multiple biomarkers. The associations between PTEN and VEGF expression status, microvessel density (MVD), and the pathological characteristics of 50 patients with ESCC were determined using χ, analysis of variance, and -tests. The results indicated that the PTEN-positive rate was negatively correlated with ESCC histological grade (P<0.01), depth of ESCC invasion (P<0.01) and lymph node metastasis status. Furthermore, the VEGF-positive rate was correlated with lymph node metastasis status, while MVD was correlated with the depth of ESCC invasion (P<0.01) and lymph node metastasis status (P<0.05). The PTEN-positive rate was negatively correlated with the VEGF-positive rate. A higher MVD was identified in ESCC samples than that of the normal esophageal mucosa, particularly in VEGF-positive ESCC specimens compared with those of VEGF-negative specimens, and PTEN-negative ESCC specimens compared with that of the PTEN-positive ESCC specimens. These results suggested that combined detection of PTEN and VEGF levels, as well as evaluation of MVD in patients with ESCC may provide essential information for improvements in the diagnosis and prognosis of ESCC.
使用单一生物标志物水平（例如磷酸酶和张力蛋白同源物（PTEN）或血管内皮生长因子（VEGF））来诊断食管鳞状细胞癌（ESCC）存在局限性。因此，本研究旨在评估联合检测多种生物标志物的临床意义。使用χ²检验、方差分析和t检验确定了50例ESCC患者的PTEN和VEGF表达状态、微血管密度（MVD）与病理特征之间的关联。结果表明，PTEN阳性率与ESCC组织学分级呈负相关（P<0.01），与ESCC浸润深度呈负相关（P<0.01）以及与淋巴结转移状态呈负相关。此外，VEGF阳性率与淋巴结转移状态相关，而MVD与ESCC浸润深度相关（P<0.01）以及与淋巴结转移状态相关（P<0.05）。PTEN阳性率与VEGF阳性率呈负相关。在ESCC样本中发现的MVD高于正常食管黏膜，特别是与VEGF阴性样本相比，VEGF阳性的ESCC标本，以及与PTEN阳性的ESCC标本相比，PTEN阴性的ESCC标本。这些结果表明，联合检测ESCC患者的PTEN和VEGF水平以及评估MVD可能为改善ESCC的诊断和预后提供重要信息。
