植物蜕皮甾酮β-蜕皮激素在啮齿动物骨髓微核试验和洋葱（Allium cepa L.）试验中具有遗传毒性。
The phytoecdysteroid β-ecdysone is genotoxic in Rodent Bone Marrow Micronuclei and Allium cepa L. Assays.
作者信息
Neves Camila Siqueira, Gomes Shaiany Sabrina Lopes, dos Santos Tatianne Rosa, de Almeida Mariana Macedo, de Souza Yamara Oliveira, Garcia Raúl Marcel González, Otoni Wagner Campos, Chedier Luciana Moreira, Viccini Lyderson Facio, de Campos José Marcello Salabert
机构信息
Universidade Federal de Juiz de Fora, Instituto de Ciências Biológicas, Departamento de Biologia, Laboratório de Genética e Biotecnologia, CEP 36036-900 Juiz de Fora, MG, Brazil.
Universidade Federal de Juiz de Fora, Instituto de Ciências Biológicas, Departamento de Biologia, Laboratório de Biologia Celular e Molecular, CEP 36036-900 Juiz de Fora, MG, Brazil.
出版信息
J Ethnopharmacol. 2016 Jan 11;177:81-4. doi: 10.1016/j.jep.2015.11.038. Epub 2015 Nov 25.
ETHNAOPHARMACOLOGIAL RELEVANCE
In South America, the β-ecdysone ecdysteroid has been found in species of the genus Pfaffia Mart. Due to the similar morphology of its roots to the Panax ginseng C. A. Mey. (Korean ginseng), some species of this genus has been known as Brazilian ginseng and have been used as tonic and aphrodisiac, as well as for the treatment of diabetes and rheumatism.
AIM OF THE STUDY
Here we report a cytogenotoxic evaluation of β-ecdysone (a natural ecdysteroid found in plants) in Rodent Bone Marrow Micronuclei and Allium cepa Assays.
MATERIALS AND METHODS
Three β-ecdysone (pure) concentrations (based in human therapeutic dosage) were used in the Micronucleus Assay. The animals were treated during two consecutive days. Micronucleated cells were counted in 2000 polychromatic erythrocytes per animal. For A. cepa L. Assay, one β-ecdysone concentration was analyzed. The onions bulbs were exposed for 24h.
RESULTS
The Micronucleus Assay showed genotoxic effects for all treatments, expressed by an increase of micronucleated cells. In A. cepa L. Assay, cell abnormalities associated to the malfunction/non-formation of mitotic spindle (aneugenic effect) and chromosomal bridges (clastogenic effect) were observed.
CONCLUSIONS
The results indicate a cytogenotoxic activity of β-ecdysone. Therefore, the popular use of Pfaffia and others species containing β-ecdysone should be considered with caution.
民族药理学相关性
在南美洲，已在Pfaffia Mart.属的物种中发现了β-蜕皮激素蜕皮甾类。由于其根部形态与五加科人参（高丽参）相似，该属的一些物种被称为巴西人参，并已被用作滋补品和壮阳药，以及用于治疗糖尿病和风湿病。
研究目的
在此，我们报告了在啮齿动物骨髓微核试验和洋葱根尖试验中对β-蜕皮激素（一种在植物中发现的天然蜕皮甾类）的细胞遗传毒性评估。
材料与方法
微核试验中使用了三种β-蜕皮激素（纯品）浓度（基于人体治疗剂量）。动物连续两天接受治疗。每只动物在2000个多色红细胞中计数微核细胞。对于洋葱根尖试验，分析了一种β-蜕皮激素浓度。洋葱鳞茎暴露24小时。
结果
微核试验显示所有处理均有遗传毒性作用，表现为微核细胞增加。在洋葱根尖试验中，观察到与有丝分裂纺锤体功能异常/未形成（非整倍体效应）和染色体桥（断裂效应）相关的细胞异常。
结论
结果表明β-蜕皮激素具有细胞遗传毒性活性。因此，应谨慎考虑Pfaffia和其他含有β-蜕皮激素的物种的普遍用途。
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