Wu Tao, Yin Jinjin, Zhang Guohua, Long Hairong, Zheng Xiaodong
Key Laboratory of Food Nutrition and Safety (Tianjin University of Science and Technology), Ministry of Education, Tianjin, PR China.
Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China.
Mol Nutr Food Res. 2016 Mar;60(3):687-94. doi: 10.1002/mnfr.201500734. Epub 2016 Jan 7.
This study aimed to determine whether cherry anthocyanin (CA) and mulberry anthocyanin (MA) can alleviate oxidative stress and inflammation associated with developing obesity in mice fed with high-fat diet (HFD).
CA and MA were added in the daily food of mice throughout the experiment. Sixty mice were randomly divided into two groups: a low-fat diet (LFD, n = 12) group and HFD (n = 48) group. Mice in the HFD group were supplied with a HFD for 8 wks to induce obesity. The HFD-fed mice were then divided into four subgroups for another 8-wk experiment. The subgroups included the control group, HFD plus Orlistat group, and HFD plus CA or MA group, with each group consisting of 12 mice. Consumption of CA and MA at 200 mg/kg food reduced bodyweight gain by 29.6 and 32.7%, respectively, in HFD-fed C57BL/6 mice. CA and MA supplementation could effectively improve the lipid profiles, decrease serum glucose and leptin levels, reduce MDA production, increase SOD and GPX activities, and down-regulate the expression of the TNFα, IL-6, iNOS, and NF-кB genes.
Therefore, MA and CA can potentially alter bodyweight by alleviating oxidative stress and inflammation in diet-induced obesity.
本研究旨在确定樱桃花青素（CA）和桑椹花青素（MA）是否能减轻高脂饮食（HFD）喂养的小鼠在肥胖发展过程中相关的氧化应激和炎症反应。
在整个实验过程中，将CA和MA添加到小鼠的日常食物中。60只小鼠随机分为两组：低脂饮食（LFD，n = 12）组和HFD（n = 48）组。HFD组的小鼠喂食HFD 8周以诱导肥胖。然后将喂食HFD的小鼠再分为四个亚组进行为期8周的实验。亚组包括对照组、HFD加奥利司他组以及HFD加CA或MA组，每组12只小鼠。在喂食HFD的C57BL/6小鼠中，以200 mg/kg食物的量摄入CA和MA分别使体重增加降低了29.6%和32.7%。补充CA和MA可有效改善血脂谱，降低血清葡萄糖和瘦素水平，减少丙二醛生成，增加超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）活性，并下调肿瘤坏死因子α（TNFα）、白细胞介素6（IL - 6）、诱导型一氧化氮合酶（iNOS）和核因子κB（NF - κB）基因的表达。
因此，MA和CA可能通过减轻饮食诱导肥胖中的氧化应激和炎症反应来改变体重。
