Li Juan, Zhang Tian-Yu, Tan Le-Tian, Wang Shu-Yi, Chen Yu-Ying, Tian Jie-Yan, Da Wen-Ying, He Ping, Zhao Ya-Ming
Department of Otolaryngology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine Shanghai 200336, China.
Department of Otolaryngology, Eye Ear Nose & Throat Hospital of Fudan University Shanghai 200031, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15521-7. eCollection 2015.
The aim of this study was to evaluate the correlation between clinical behavior and expression of human papillomavirus (HPV) in patients with juvenile laryngeal papillomatosis, in an attempt to develop an effective molecular biological method to predict prognosis. We included 37 patients with juvenile laryngeal papillomatosis in the study group and 10 cases each of juvenile vocal cord polyps and juvenile normal laryngeal mucosa as the control group. We detected HPV by immunocytochemistry and in situ hybridization, identified the virus type, and measured HPV-DNA content using a computer-assisted, color pathological image-analysis system. Additionally, we conducted a retrospective study with regard to the patients' clinical history to evaluate the prognosis. The data of the 2 groups were compared and statistically analyzed, including a correlation with prognosis. In the study group, 67.3% (25/37) were positive for HPV-Ag by immunocytochemistry; whereas 53.2%, 45.8%, and 25.4% were positive for HPV6b-DNA, HPV11-DNA, and HPV6b+11-DNA, respectively, by in situ hybridization. HPV was not detected in the control group. There was a significant difference between two groups (P < 0.05). Compared to HPV11-DNA-positive cases, those that were positive for HPV6b-DNA and HPV6b+11-DNA showed lower results on average, for age at first diagnosis and self-relief, number of surgeries, and interval between surgeries. Our findings suggest that immunocytochemistry and in situ hybridization are useful methods to evaluate the prognosis of juvenile laryngeal papilloma (JLP) and that HPV6b-positivity can be used as an index to predict the development and outcome of JLP.
本研究旨在评估青少年喉乳头状瘤患者的临床行为与人类乳头瘤病毒（HPV）表达之间的相关性，以期开发一种有效的分子生物学方法来预测预后。研究组纳入37例青少年喉乳头状瘤患者，对照组纳入10例青少年声带息肉患者和10例青少年正常喉黏膜患者。我们采用免疫细胞化学和原位杂交技术检测HPV，鉴定病毒类型，并使用计算机辅助彩色病理图像分析系统测量HPV-DNA含量。此外，我们对患者的临床病史进行了回顾性研究以评估预后。对两组数据进行比较和统计分析，包括与预后的相关性。研究组中，免疫细胞化学检测HPV-Ag阳性率为67.3%（25/37）；而原位杂交检测HPV6b-DNA、HPV11-DNA和HPV6b+11-DNA阳性率分别为53.2%、45.8%和25.4%。对照组未检测到HPV。两组之间存在显著差异（P<0.05）。与HPV11-DNA阳性病例相比，HPV6b-DNA和HPV6b+11-DNA阳性病例在首次诊断年龄、自行缓解情况、手术次数以及手术间隔时间等方面的平均结果较低。我们的研究结果表明，免疫细胞化学和原位杂交是评估青少年喉乳头状瘤（JLP）预后的有用方法，HPV6b阳性可作为预测JLP发展和转归的指标。
