Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China; Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, PR China.
Biosens Bioelectron. 2016 Apr 15;78:308-314. doi: 10.1016/j.bios.2015.11.063. Epub 2015 Nov 23.
In this work, we developed a novel freestanding metallic microrod as working electrode for highly sensitive and selective electrochemical detection of trace dopamine (DA). The electrode was facilely fabricated via first dealloying smooth Au-Ag alloy microrod (AMR) into nanoporous Au-Ag alloy microrod (NPAMR) and further modifying with electro-polymerized molecularly imprinted polymer (MIP). Influencing factors during electro-polymerization process including pH value and molar ratio of monomer to template molecule were optimized. Under the optimal conditions, a linear range from 2 × 10(-13) to 2 × 10(-8)M for measuring DA was obtained with an ultralow detection limit of 7.63 × 10(-14)M (S/N=3). In addition, the MIP-modified electrode (MIP/NPAMR) was successfully employed to test DA in serum and brain samples.
在这项工作中，我们开发了一种新型的独立式金属微棒作为工作电极，用于痕量多巴胺（DA）的高灵敏度和选择性电化学检测。该电极通过首先将光滑的 Au-Ag 合金微棒（AMR）脱合金化为纳米多孔 Au-Ag 合金微棒（NPAMR），然后进一步用电聚合分子印迹聚合物（MIP）进行修饰来制备。优化了电聚合过程中的影响因素，包括 pH 值和单体与模板分子的摩尔比。在最佳条件下，用于测量 DA 的线性范围为 2×10(-13)至 2×10(-8)M，检测限低至 7.63×10(-14)M（S/N=3）。此外，该修饰电极（MIP/NPAMR）成功用于血清和脑样中 DA 的检测。
