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Brief report of the construction of infectious DNA clones of South African genetic variants of grapevine virus A and grapevine virus B.

作者信息

Goszczynski D E

机构信息

Plant Protection Research Institute, Agricultural Research Council, Private Bag X134, Queenswood, Pretoria, 0121 South Africa.

出版信息

Springerplus. 2015 Nov 26;4:739. doi: 10.1186/s40064-015-1517-2. eCollection 2015.

DOI:10.1186/s40064-015-1517-2
PMID:26640751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4661162/
Abstract

BACKGROUND

Recent research results strongly suggest that certain genetic variants of grapevine virus A (GVA) and grapevine virus B (GVB), two members of the Vitivirus genus of the family Betaflexiviridae, are the cause of Shiraz disease and corky bark disease of grapevines in South Africa, respectively. To investigate this hypothesis, work was undertaken to construct DNA clones of these viruses.

FINDINGS AND CONCLUSIONS

Biologically viable and stable DNA clones of genetic variants of GVA and GVB B from South Africa were constructed. The clones share 76.3, 73.2 and 85.2, 77.6 % nt sequence similarity with corresponding clones constructed in Italy and Israel. The results suggest that a derivative of a mini binary vector pCB302 is superior to pCAMBIA1305.1 for the construction of infectious and stable DNA clones of vitiviruses. Successful construction of such DNA clones of GVA and GVB reported in this study is a clear step towards fulfilling Koch's 3rd postulate in investigating the aetiology of Shiraz disease and corky bark disease.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/db2e1683f0b7/40064_2015_1517_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/1bf2f0f0274c/40064_2015_1517_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/fb8a34e39420/40064_2015_1517_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/db2e1683f0b7/40064_2015_1517_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/1bf2f0f0274c/40064_2015_1517_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/fb8a34e39420/40064_2015_1517_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eca5/4661162/db2e1683f0b7/40064_2015_1517_Fig3_HTML.jpg

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本文引用的文献

1
Genetic diversity of Grapevine virus A in Washington and California vineyards.华盛顿和加利福尼亚葡萄园中葡萄扇叶病毒 A 的遗传多样性。
Phytopathology. 2014 May;104(5):548-60. doi: 10.1094/PHYTO-06-13-0179-R.
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Virus-derived gene expression and RNA interference vector for grapevine.葡萄病毒衍生基因表达和 RNA 干扰载体。
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Divergent molecular variants of Grapevine virus B (GVB) from corky bark (CB)-affected and CB-negative LN33 hybrid grapevines.
来自受栓皮病（CB）影响和CB阴性的LN33杂交葡萄藤的葡萄病毒B（GVB）的不同分子变体。
Virus Genes. 2010 Oct;41(2):273-81. doi: 10.1007/s11262-010-0511-6. Epub 2010 Jul 14.
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Class XI myosins are required for development, cell expansion, and F-Actin organization in Arabidopsis.Class XI 肌球蛋白在拟南芥的发育、细胞扩张和 F-肌动蛋白组织中是必需的。
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Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera.葡萄病毒A介导的本氏烟草和葡萄中的基因沉默
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Molecular divergence of Grapevine virus A (GVA) variants associated with Shiraz disease in South Africa.与南非设拉子病相关的葡萄病毒A（GVA）变种的分子差异
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Cloning of large positive-strand RNA viruses.大型正链RNA病毒的克隆
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Sequencing and assembly of a full-length infectious clone of grapevine virus B and its infectivity on herbaceous plants.
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.MEGA4：分子进化遗传学分析（MEGA）软件版本4.0。
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Family Flexiviridae: a case study in virion and genome plasticity.柔线病毒科：病毒粒子与基因组可塑性的一个案例研究
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