Del Campo Cristian, Ignatova Zoya
Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Hamburg, Germany.
Curr Genet. 2016 May;62(2):331-4. doi: 10.1007/s00294-015-0551-5. Epub 2015 Dec 9.
mRNA is a nexus entity between DNA and translating ribosomes. Recent developments in deep sequencing technologies coupled with structural probing have revealed new insights beyond the classic role of mRNA and place it more centrally as a direct effector of a variety of processes, including translation, cellular localization, and mRNA degradation. Here, we highlight emerging approaches to probe mRNA secondary structure on a global transcriptome-wide level and compare their potential and resolution. Combined approaches deliver a richer and more complex picture. While our understanding on the effect of secondary structure for various cellular processes is quite advanced, the next challenge is to unravel more complex mRNA architectures and tertiary interactions.
信使核糖核酸（mRNA）是DNA与进行翻译的核糖体之间的关键实体。深度测序技术与结构探测技术的最新进展揭示了超越mRNA经典作用的新见解，并将其更核心地定位为包括翻译、细胞定位和mRNA降解在内的多种过程的直接效应物。在这里，我们重点介绍在全球转录组水平上探测mRNA二级结构的新兴方法，并比较它们的潜力和分辨率。综合方法提供了更丰富、更复杂的图景。虽然我们对二级结构对各种细胞过程的影响的理解已经相当深入，但下一个挑战是解开更复杂的mRNA结构和三级相互作用。
