Pitkin D H, Actor P, Baldinus J G, Post A, Weisbach J A
J Antibiot (Tokyo). 1978 Apr;31(4):359-62. doi: 10.7164/antibiotics.31.359.
A quantitative semi-automated turbidimetric bioassay for cefazaflur, using Streptococcus faecium as the indicator, is described. Assays were run at pH 6.5 approximately 7 for 3.75 hours at 37 degrees C using 2 approximately 12 microgram cefazaflur per ml assay broth for standards. The dose response line was plotted point to point using the natural log of the absorbance vs natural log of the concentration. This assay is both accurate and precise and is more rapid than traditional plate assays for antibiotics.
描述了一种使用粪肠球菌作为指示剂的头孢唑氟定量半自动比浊生物测定法。测定在pH 6.5约7的条件下，于37℃运行3.75小时，标准品使用每毫升测定肉汤中含2约12微克头孢唑氟。剂量反应线通过吸光度的自然对数与浓度的自然对数逐点绘制。该测定法既准确又精确，并且比传统的抗生素平板测定法更快。
