Rippere R A
J Assoc Off Anal Chem. 1979 Jul;62(4):951-6.
Turbidimetric methods for determining the potency of antibiotics are inherently more accurate and more precise than are comparable agar diffusion procedures, but assays conducted in liquid media are subject to degradation from less than ideal conditions to a much greater extent than are diffusion methods. The relationships between test organisms, antibiotics, and assay concentrations are discussed. A valid assay procedure must produce a linear response with an adequate slope (-0.4 to -1.2) by the test organism to increasing concentrations of drug; such linear response normally occurs over a limited range of concentrations. Criteria used to select photometers that offer the greatest advantages to analytical microbiologists are described, with guidelines for the most effective use of the chosen instrument.
用于测定抗生素效价的比浊法本质上比类似的琼脂扩散法更准确、更精密，但在液体培养基中进行的测定比扩散法更容易受到不理想条件的影响而降解。文中讨论了测试微生物、抗生素和测定浓度之间的关系。有效的测定程序必须使测试微生物对药物浓度增加产生具有适当斜率（-0.4至-1.2）的线性响应；这种线性响应通常发生在有限的浓度范围内。文中描述了用于选择对分析微生物学家最具优势的光度计的标准，以及所选仪器最有效使用的指南。
