Ohgushi H, Goldberg V M, Caplan A I
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
Acta Orthop Scand. 1989 Jun;60(3):334-9. doi: 10.3109/17453678909149289.
We studied the role of bone-marrow reconstituted porous ceramics in enhancing healing of a 5-mm femoral diaphyseal defect fixed with a rigid polyethylene plate in rats. Osseous repair was evaluated by histologic scoring. When blocks of porous calcium phosphate ceramics alone were introduced into the defects, most cases showed fibrous tissue interposition at the host bone-ceramic junction 1 month after implantation, and only four of 12 defects developed osseous or osteochondral union at both the proximal and distal junctions 2 months after surgery. However, when the ceramic was combined with syngeneic viable marrow cells, new bone formation occurred in isolated pore regions of the ceramic at 1 month, and extensive bone formation was seen in most pore regions 2 months after implantation. Out of 12 implants, complete bone union was seen in eight, and one showed osseous or osteochondral union at both junctions 2 months after surgery. Our results indicated that composite grafts of porous calcium phosphate ceramics and marrow cells may be clinically applicable to enhance osteogenesis and osteoconduction.
我们研究了骨髓重建多孔陶瓷在促进大鼠股骨干5毫米骨缺损愈合中的作用，该缺损用刚性聚乙烯板固定。通过组织学评分评估骨修复情况。当仅将多孔磷酸钙陶瓷块植入缺损时，大多数病例在植入1个月后在宿主骨与陶瓷交界处出现纤维组织插入，术后2个月12个缺损中只有4个在近端和远端交界处形成骨或骨软骨联合。然而，当陶瓷与同基因活骨髓细胞联合使用时，1个月时在陶瓷的孤立孔隙区域出现新骨形成，植入2个月后在大多数孔隙区域可见广泛的骨形成。12个植入物中，8个实现了完全骨愈合，1个在术后2个月在两个交界处均出现骨或骨软骨联合。我们的结果表明，多孔磷酸钙陶瓷与骨髓细胞的复合移植物可能在临床上适用于增强成骨作用和骨传导。
