Magnússon Skúli, Baldursson Baldur Tumi, Kjartansson Hilmar, Thorlacius Guðný Ella, Axelsson Ívar, Rolfsson Óttar, Petersen Pétur Henry, Sigurjónsson Guðmundur Fertram
Laeknabladid. 2015 Dec;101(12):567-73. doi: 10.17992/lbl.2015.12.54.
Acellular fish skin of the Atlantic cod (Gadus morhua) is being used to treat chronic wounds. The prevalence of diabetes and the comorbidity of chronic wounds is increasing globally. The aim of the study was to assess the biocompatibility and biological characteristics of acellular fish skin, important for tissue repair.
The structure of the acellular fish skin was examined with microscopy. Biocompatibility of the graft was conducted by a specialized certified laboratory. Protein extracts from the material were analyzed using gel electrophoresis. Cytokine levels were measured with an enzyme linked immunosorbent assay (ELISA). Angiogenic properties were assessed with a chick chorioallantoic membrane (chick CAM) assay.
The structure of acellular fish skin is porous and the material is biocompatible. Electrophoresis revealed proteins around the size 115-130 kDa, indicative of collagens. The material did not have significant effect on IL-10, IL-12p40, IL-6 or TNF-α secretion from monocytes or macrophages. Acellular fish skin has significant effect on angiogenesis in the chick CAM assay.
The acellular fish skin is not toxic and is not likely to promote inflammatory responses. The graft contains collagen I, promotes angiogenesis and supports cellular ingrowth. Compared to similar products made from mammalian sources, acellular fish skin does not confer a disease risk and contains more bioactive compounds, due to less severe processing.
大西洋鳕鱼（Gadus morhua）的脱细胞鱼皮正被用于治疗慢性伤口。全球范围内，糖尿病的患病率以及慢性伤口的合并症正在增加。本研究的目的是评估对于组织修复很重要的脱细胞鱼皮的生物相容性和生物学特性。
用显微镜检查脱细胞鱼皮的结构。移植物的生物相容性由一家专业认证实验室进行检测。使用凝胶电泳分析该材料的蛋白质提取物。用酶联免疫吸附测定法（ELISA）测量细胞因子水平。用鸡胚绒毛尿囊膜（鸡胚CAM）试验评估血管生成特性。
脱细胞鱼皮的结构是多孔的，且该材料具有生物相容性。电泳显示大小在115 - 130 kDa左右的蛋白质，表明存在胶原蛋白。该材料对单核细胞或巨噬细胞分泌IL - 10、IL - 12p40、IL - 6或TNF - α没有显著影响。在鸡胚CAM试验中，脱细胞鱼皮对血管生成有显著影响。
脱细胞鱼皮无毒，且不太可能促进炎症反应。该移植物含有I型胶原蛋白，能促进血管生成并支持细胞向内生长。与源自哺乳动物的类似产品相比，脱细胞鱼皮不会带来疾病风险，并且由于加工过程不太严格，含有更多生物活性化合物。
