Magnusson Skuli, Baldursson Baldur Tumi, Kjartansson Hilmar, Rolfsson Ottar, Sigurjonsson Gudmundur Fertram
Kerecis Limited, Eyrargata 2, 400 Isafjordur, Iceland.
Center for Systems Biology, University of Iceland, Sturlugata 8, 101 Reykjavik, Iceland.
Mil Med. 2017 Mar;182(S1):383-388. doi: 10.7205/MILMED-D-16-00142.
Improvised explosive devices and new directed energy weapons are changing warfare injuries from penetrating wounds to large surface area thermal and blast injuries. Acellular fish skin is used for tissue repair and during manufacturing subjected to gentle processing compared to biologic materials derived from mammals. This is due to the absence of viral and prion disease transmission risk, preserving natural structure and composition of the fish skin graft.
The aim of this study was to assess properties of acellular fish skin relevant for severe battlefield injuries and to compare those properties with those of dehydrated human amnion/chorion membrane.
We evaluated cell ingrowth capabilities of the biological materials with microscopy techniques. Bacterial barrier properties were tested with a 2-chamber model.
The microstructure of the acellular fish skin is highly porous, whereas the microstructure of dehydrated human amnion/chorion membrane is mostly nonporous. The fish skin grafts show superior ability to support 3-dimensional ingrowth of cells compared to dehydrated human amnion/chorion membrane (p < 0.0001) and the fish skin is a bacterial barrier for 24 to 48 hours.
The unique biomechanical properties of the acellular fish skin graft make it ideal to be used as a conformal cover for severe trauma and burn wounds in the battlefield.
简易爆炸装置和新型定向能武器正在改变战争创伤，使其从穿透伤转变为大面积热烧伤和爆炸伤。与源自哺乳动物的生物材料相比，脱细胞鱼皮用于组织修复，且在制造过程中受到的处理较为温和。这是因为不存在病毒和朊病毒疾病传播风险，能保留鱼皮移植物的天然结构和成分。
本研究旨在评估与严重战场创伤相关的脱细胞鱼皮的特性，并将这些特性与脱水人羊膜/绒毛膜的特性进行比较。
我们用显微镜技术评估了生物材料的细胞向内生长能力。用双室模型测试了细菌屏障特性。
脱细胞鱼皮的微观结构高度多孔，而脱水人羊膜/绒毛膜的微观结构大多无孔。与脱水人羊膜/绒毛膜相比，鱼皮移植物显示出更强的支持细胞三维向内生长的能力（p < 0.0001），并且鱼皮在24至48小时内是细菌屏障。
脱细胞鱼皮移植物独特的生物力学特性使其非常适合用作战场严重创伤和烧伤创面的贴合覆盖物。
