Szcześniak Michał W, Rosikiewicz Wojciech, Makałowska Izabela
Department of Bioinformatics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, 61-614 Poznan, Poland
Department of Bioinformatics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, 61-614 Poznan, Poland.
Plant Cell Physiol. 2016 Jan;57(1):e8. doi: 10.1093/pcp/pcv201. Epub 2015 Dec 12.
Long non-coding RNAs (lncRNAs) represent a class of potent regulators of gene expression that are found in a wide array of eukaryotes; however, our knowledge about these molecules in plants is still very limited. In particular, a number of model plant species still lack comprehensive data sets of lncRNAs and their annotations, and very little is known about their biological roles. To meet these shortcomings, we created an online database of lncRNAs in 10 model plant species. The lncRNAs were identified computationally using dozens of publicly available RNA sequencing (RNA-Seq) libraries. Expression values, coding potential, sequence alignments as well as other types of data provide annotation for the identified lncRNAs. In order to better characterize them, we investigated their potential roles in splicing modulation and deregulation of microRNA functions. The data are freely available for searching, browsing and downloading from an online database called CANTATAdb (http://cantata.amu.edu.pl, http://yeti.amu.edu.pl/CANTATA/).
长链非编码RNA(lncRNAs)是一类在多种真核生物中发现的基因表达强效调节因子;然而,我们对植物中这些分子的了解仍然非常有限。特别是,许多模式植物物种仍然缺乏lncRNAs的全面数据集及其注释,对它们的生物学作用也知之甚少。为了弥补这些不足,我们创建了一个包含10种模式植物lncRNAs的在线数据库。使用数十个公开可用的RNA测序(RNA-Seq)文库通过计算鉴定lncRNAs。表达值、编码潜能、序列比对以及其他类型的数据为鉴定出的lncRNAs提供注释。为了更好地表征它们,我们研究了它们在剪接调控和微小RNA功能失调中的潜在作用。这些数据可从名为CANTATAdb的在线数据库(http://cantata.amu.edu.pl,http://yeti.amu.edu.pl/CANTATA/)免费搜索、浏览和下载。