hup基因产物参与大肠杆菌中的位点特异性DNA倒位。

Participation of the hup gene product in site-specific DNA inversion in Escherichia coli.

作者信息

Wada M, Kutsukake K, Komano T, Imamoto F, Kano Y

机构信息

Laboratory of Molecular Genetics, Riken, Tsukuba Life Science Center, Ibaraki, Japan.

出版信息

Gene. 1989;76(2):345-52. doi: 10.1016/0378-1119(89)90174-1.

DOI:10.1016/0378-1119(89)90174-1

PMID:2666260

Abstract

The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.

摘要

密切相关的大肠杆菌基因hupA和hupB各自编码一种细菌类组蛋白HU。我们在此报告，由hin、gin、pin和rci介导而非cin介导的DNA倒位在hupA hupB双突变体中受阻，尽管这些系统中的倒位在hupA或hupB单突变体中与在野生型菌株中一样高效地发生。这些发现表明，HU蛋白参与大肠杆菌中的位点特异性DNA倒位，并且仅一个亚基，即HU-1或HU-2，就足以进行这种倒位。